Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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On the binding of ATP to the autophosphorylating protein, Ptk, of the bacterium Acinetobacter johnsonii.
TL;DR: It is concluded that even though the overall autophosphorylation reaction is similar in eukaryotic and prokaryotic proteins, the mechanism involved is likely different.
Journal ArticleDOI
Identification and expression of a cloned yeast heat shock gene.
D B Finkelstein,S Strausberg +1 more
TL;DR: The presence of an increased dosage of the HSP90 gene has no effect on the synthesis of the other major heat shock-inducible proteins and does not alter the heatshock-associated phenotype of thermal tolerance.
Journal ArticleDOI
Deletion analysis of the F plasmid oriT locus.
TL;DR: Data show that the F oriT locus is extensive, with domains that individually contribute to transfer, nicking, and overall structure, indicating that phase relationships between nicking and transfer domains of oriT must be preserved for full oriT function.
Journal ArticleDOI
Autophosphorylation of Phosphoglucosamine Mutase from Escherichia coli
TL;DR: It is shown that the phosphoglucosamine mutase from Escherichia coli autophosphorylates in vitro in the presence of [(32)P]ATP and analysis of enzyme phosphorylation by high-pressure liquid chromatography and coupled mass spectrometry confirms that only one phosphate has been covalently linked to the enzyme.
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Characterization of class 1 integron resistance gene cassettes and the identification of a novel IS-like element in Acinetobacter baumannii.
Heidi Segal,Robin Thomas +1 more
TL;DR: Based on hybridization studies, 21/32 multi-resistant clinical isolates of Acinetobacter baumannii contain class 1 integrons and DNA sequence analysis of a 6.080-kb fragment identified the remnant of an integron, following insertion of IS26 into the 5(')-end of intI1.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.