Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
Citations
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Restriction map of the Escherichia coli malA region and identification of the malT product.
O Raibaud,M Schwartz +1 more
TL;DR: Estimates of the size of the malT gene support the conclusion that the 94,000-dalton polypeptide which was not produced by identical strains carrying similar plasmids in which malT was partially deleted is themalT product.
Journal ArticleDOI
Nucleotide sequence of the guaB locus encoding IMP dehydrogenase of Escherichia coli K12.
Amelia A. Tiedeman,John M. Smith +1 more
TL;DR: IMP dehydrogenase, the product of the guaB locus in Escherichia coli K12, catalyzes the synthesis of XMP by the NAD+ dependent oxidation of IMP.
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Isolation and characterization of faithful and altered clones of the genomes of cauliflower mosaic virus isolates Cabb B-JI, CM4-184, and Bari I.
Michel Delseny,Roger Hull +1 more
TL;DR: All the deletions overlap one or two of the specific single-stranded breaks characteristic of caulimoviruses, suggesting that sequences surrounding the breaks are not dispensable.
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Cloning, functional expression and promoter analysis of xylanase III gene from Trichoderma reesei
Wataru Ogasawara,Yosuke Shida,Takanori Furukawa,R Shimada,S Nakagawa,M Kawamura,T Yagyu,A Kosuge,J Xu,Masahiro Nogawa,Hirofumi Okada,Yasushi Morikawa +11 more
TL;DR: The results suggest that xyn3 expression in T. reesei QM9414 is silenced, and the deduced amino acid sequence of XYN III revealed considerable homology with xylanases belonging to glycoside hydrolase family 10.
Journal ArticleDOI
Seventeen base pairs of region I encode a novel tripartite binding signal for SV40 T antigen.
Kevin Ryder,Evangelia Vakalopoulou,Ronald Mertz,Iris A. Mastrangelo,Paul V. C. Hough,P Tegtmeyer,Ellen Fanning +6 more
TL;DR: It is proposed that any two of the three sequence signals are sufficient to determine the unique arrangement of a bound protein dimer.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.