Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Cloning and structure analysis of the rat apolipoprotein A-I cDNA
TL;DR: Comparison of the deduced protein sequence with its human counterpart reveals a striking homology between the prepropeptide precursors, suggesting that this particular domain could be involved in lipid/protein binding or lecithin-cholesterol acyltransferase activation.
Journal ArticleDOI
Genetic system for analyzing Escherichia coli thymidylate synthase.
TL;DR: Random in vitro mutagenesis of the thyA gene is being used to delineate its regulatory elements as well as the functional domains of its product, thymidylate synthase, which permitted the isolation of 400 mutants, which are being categorized by phenotypic and genetic criteria.
Journal ArticleDOI
Regions of DNA involved in the stringent control of plasmid-encoded rRNA in vivo
TL;DR: It is found that rRNA synthesized from plasmids does exhibit a relA-dependent, stringent response and the presence of extra intact rrnB operons (carried by a multicopy plasmid) reduces the magnitude of the stringent response.
Journal ArticleDOI
Identification of a positive regulator of the Mu middle operon.
Kalai Mathee,M M Howe +1 more
TL;DR: The mor requirement for plaque formation and normal growth kinetics was abolished when C protein was provided in trans, indicating that the primary function of Mor is to provide sufficient C for late gene expression.
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Specific pattern of instability of Escherichia coli HisG gene cloned in Bacillus subtilis via the Staphylococcus aureus plasmid pCS194
TL;DR: The plasmid pCS194, generated in vivo by recombination of two Staphylococcus aureus plasmids, pC194 and pS194, coding, respectively, for chloramphenicol and streptomycin resistance, can be replicated also in Bacillus subtilis in the presence of either of the two antibiotics.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.