Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
Citations
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Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens
TL;DR: It is concluded that polymorphic determinants recognized by monoclonal antibodies are located in functionally distinct portions of the transplantation antigen protein.
Journal ArticleDOI
The divIVA minicell locus of Bacillus subtilis.
Jeong Heon Cha,George C. Stewart +1 more
TL;DR: The data support a model in which divIVA encodes the topological specificity subunit of the minCD system, and suggests that DivIVA acts as a pilot protein, directing minCD to the polar septation sites of the sporulation process.
Journal ArticleDOI
Genetically manipulated virulence of Yersinia enterocolitica.
TL;DR: The results demonstrate that plasmid-mediated animal virulence functions are not uniformly expressed within Y. enterocolitica.
Journal ArticleDOI
Serum vitamin D-binding protein is a third member of the albumin and alpha fetoprotein gene family.
N E Cooke,E V David +1 more
TL;DR: A near full-length cDNA encoding the human vitamin D-binding protein (hDBP) was isolated from a human liver mRNA expression library and it is established that DBP is a member of the ALB and AFP gene family.
Journal ArticleDOI
Partition of unit-copy miniplasmids to daughter cells: III. The DNA sequence and functional organization of the P1 partition region
TL;DR: The boundaries of the P1 par (plasmid partition) region of the unit-copy plasmid P1 were defined to within 2.7 X 10(3) base-pairs of DNA, revealing two large open reading frames that could encode proteins of Mr 44,000 and Mr 38,000.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.