Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
Reads0
Chats0
TLDR
Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
Citations
More filters
Journal ArticleDOI
Physical mapping of transposon Tn5 insertions defines a gene cluster functional in nitrous oxide respiration by Pseudomonas stutzeri.
A Viebrock,Walter G. Zumft +1 more
TL;DR: By transposon Tn5 mutagenesis, 19 strains of Pseudomonas stutzeri were acquired that had defects in nitrous oxide respiration (Nos- phenotype), and a physical map of the mutants showed nearly random Tn 5 insertions into genomic DNA within a single region ca.
Journal ArticleDOI
Identification of a promoter sequence in the plasmid pUL340 of Brevibacterium lactofermentum and construction of new cloning vectors for corynebacteria containing two selectable markers.
TL;DR: A strong promoter P1 has been found in plasmid pUL340, a cloning vector used to transform corynebacteria, and provides useful screening markers for selecting transformants of B. lactofermentum together with the kanamycin-resistance gene from the transposon Tn5.
Journal ArticleDOI
Detection and characterization by differential PCR of host eukaryotic cell genes differentially transcribed following uptake of intracellular bacteria.
TL;DR: The results demonstrate that DPCR can identify specific host cell genes which are differentially transcribed after infection with certain microorganisms and some of these genes may be new or may never before have been linked to interactions between hosts and pathogens.
Journal ArticleDOI
Molecular cloning and expression of a locus (mdoA) implicated in the biosynthesis of membrane-derived oligosaccharides in Escherichia coli.
TL;DR: Mutants of Escherichia coli defective in the mdoA locus are blocked at an early stage in the biosynthesis of membrane‐derived oligosaccharides and has now been cloned into multicopy plasmids.
Journal ArticleDOI
Structure of chicken calcitonin predicted by partial nucleotide sequence of its precursor.
TL;DR: The partial nucleotide sequence of chicken calcitonin mRNA and the deduced complete amino acid sequence of Chicken Calcitonin are reported here.
References
More filters
Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.