Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
Reads0
Chats0
TLDR
Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
Citations
More filters
Journal ArticleDOI
Transposable elements of Xanthomonas campestris pv. citri originating from indigenous plasmids
Jenn Tu,Huei-Rong Wang,Shau-Feng Chang,Yuh-Chyang Charng,Rudi Lurz,Beate Dobrinski,Wen-Chuan Wu +6 more
TL;DR: Restriction analysis, DNA hybridization and heteroduplex experiments revealed that ISXC4 andISXC5 are about 5.55 and 6.95 kb in size, respectively, and possess inverted repeats about 50±18 bp in length and share DNA homology in their left and right ends.
Journal ArticleDOI
Molecular cloning of a gene coding for a Vibrio cholerae haemagglutinin
TL;DR: Both Escherichia coli HB101 containing the recombinant plasmids and V. cholerae C5 were able to agglutinate a variety of erythrocytes from human and animal origin; this haemagglutination was not inhibited by D-mannose or L-fucose.
Journal ArticleDOI
Isolation of an autonomously replicating DNA fragment from the region of defective bacteriophage PBSX of Bacillus subtilis.
L M Anderson,H E Ruley,K F Bott +2 more
TL;DR: It is believed that, like E. coli, B. subtilis contains at least one DNA fragment capable of autonomous replication when liberated from its normally integrated chromosomal site and that this cloned DNA fragment comes from the region of defective bacteriophage PBSX.
Journal ArticleDOI
Bacteriophage Mu Mor protein requires sigma 70 to activate the Mu middle promoter
Kalai Mathee,Martha M. Howe +1 more
TL;DR: The sigma 70 dependence of Mor-specific transcription and the amino acid sequence similarity between Mor and C (an activator for Mu late transcription) both support the hypothesis that Mor functions mechanistically as an activator protein.
Journal ArticleDOI
Isolation of genes preferentially expressed during Bacillis subtilis spore outgrowth.
TL;DR: Using the cloned DNAs and derived plasmids in dot hybridization experiments with RNA extracted from cells at different developmental stages, a region that is transcribed only during the outgrowth phase is defined for two clones.
References
More filters
Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.