Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Improved production of 3-hydroxypropionic acid in engineered Escherichia coli by rebalancing heterologous and endogenous synthetic pathways
TL;DR: A novel E. coli strain is constructed that overexpresses the endogenous aldehyde dehydrogenase gene (puuC) under the control of a strong promoter and inactivated the puu operon repressor gene, puuR, which resulted in a decrease in glycerol accumulation and an increase in 3-HP production through the co-fermentation of glucose and xylose.
Journal ArticleDOI
Deciphering the role of the type II glyoxalase isoenzyme YcbL (GlxII-2) in Escherichia coli
TL;DR: It is shown that YcbL from Salmonella enterica serovar Typhimurium acts as an accessory type II glyoxalase in E. coli, which has additive effects and ensures maximal MG degradation.
Dissertation
Cloning and characterisation of cDNAs encoding the major, pea storage proteins, and expression of vicilin in E. coli
TL;DR: Comparisons of protein and cDNA-encoded amino acid sequences indicated that vicilins are synthesised as polypeptide precursors which subsequently undergo the removal of an N-terminal signal pep tide and possibly a C-terminals extension, as well as being susceptible to endo-proteolytic processing.
Journal ArticleDOI
A silent regulatory gene cfaD' on region 1 of the CFA/I plasmid NTP 113 of enterotoxigenic Escherichia coli.
Wim Gaastra,Bart J.A.M. Jordi,Emmy M.A. Mul,Anja M. Harriers,Moyra M. McConnell,Geraldine A. Willshaw,Henry R. Smith,Bernard A. M. van der Zeijst +7 more
TL;DR: A DNA sequence, homologous to the cfaD gene of CFA/I region 2, was identified on CFA-I region 1, which can only encode a truncated CfaD-like protein and may be a DNA binding protein and functions as a positive regulator of C FA/I fimbriae expression.
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Escherichia coli membrane vesicles with elevated phosphatidic acid levels. A detergent-free system for in vitro phospholipid synthesis.
TL;DR: It is shown that phosphatidic acid that accumulates in E. coli cytidine auxotrophs is competent for further biosynthetic use in vivo, if cytidine is re-supplied to the cells.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.