Journal ArticleDOI
The complete DNA sequence of yeast chromosome III.
Stephen G. Oliver,Q. J. M. van der Aart,M. L. Agostoni-Carbone,Michel Aigle,Lilia Alberghina,Despina Alexandraki,G. Antoine,Rashida Anwar,Juan P. G. Ballesta,Paule Bénit,Gilbert Berben,Elisabetta Bergantino,N. Biteau,P. A. Bolle,Monique Bolotin-Fukuhara,Anthony G. A. Brown,Alistair J. P. Brown,J. M. Buhler,C. Carcano,Giovanna Carignani,Håkan Cederberg,R. Chanet,Roland Contreras,Marc Crouzet,B. Daignan-Fornier,E. Defoor,M. Delgado,Jan Demolder,C. Doira,Evelyne Dubois,Bernard Dujon,A. Düsterhöft,D. Erdmann,M. Esteban,F. Fabre,Cécile Fairhead,Gérard Faye,Horst Feldmann,Walter Fiers,M. C. Francingues-Gaillard,L. Franco,Laura Frontali,H. Fukuhara,L. J. Fuller,P. Galland,Manda E. Gent,D. Gigot,Véronique Gilliquet,Glansdorff Nn,André Goffeau,M. Grenson,P. Grisanti,Leslie A. Grivell,M. de Haan,M. Haasemann,D. Hatat,Janet Hoenicka,Johannes H. Hegemann,C. J. Herbert,François Hilger,Stefan Hohmann,Cornelis P. Hollenberg,K. Huse,F. Iborra,K. J. Indje,K. Isono,C. Jacq,M. Jacquet,C. M. James,J. C. Jauniaux,Y. Jia,Alberto Jiménez,A. Kelly,U. Kleinhans,P Kreisl,G. Lanfranchi,C Lewis,C. G. vanderLinden,G Lucchini,K Lutzenkirchen,M.J. Maat,L. Mallet,G. Mannhaupet,Enzo Martegani,A. Mathieu,C. T. C. Maurer,David J. McConnell,R. A. McKee,F. Messenguy,Hans-Werner Mewes,Francis Molemans,M. A. Montague,M. Muzi Falconi,L. Navas,Carol S. Newlon,D. Noone,C. Pallier,L. Panzeri,Bruce M. Pearson,J. Perea,Peter Philippsen,A. Pierard,Rudi J. Planta,Paolo Plevani,B. Poetsch,Fritz M. Pohl,B. Purnelle,M. Ramezani Rad,S. W. Rasmussen,A. Raynal,Miguel Remacha,P. Richterich,Aki Roberts,F. Rodriguez,E. Sanz,I. Schaaff-Gerstenschlager,Bart Scherens,Bertold Schweitzer,Y. Shu,J. Skala,Piotr P. Slonimski,F. Sor,C. Soustelle,R. Spiegelberg,Lubomira Stateva,H. Y. Steensma,S. Steiner,Agnès Thierry,George Thireos,Maria Tzermia,L. A. Urrestarazu,Giorgio Valle,I. Vetter,J. C. van Vliet-Reedijk,Marleen Voet,Guido Volckaert,P. Vreken,H. Wang,John R. Warmington,D. von Wettstein,Barton Luke Wicksteed,C. Wilson,H. Wurst,G. Xu,A. Yoshikawa,Friedrich K. Zimmermann,J. G. Sgouros +146 more
TLDR
The entire DNA sequence of chromosome III of the yeast Saccharomyces cerevisiae has been determined, which is the first complete sequence analysis of an entire chromosome from any organism.Abstract:
The entire DNA sequence of chromosome III of the yeast Saccharomyces cerevisiae has been determined. This is the first complete sequence analysis of an entire chromosome from any organism. The 315-kilobase sequence reveals 182 open reading frames for proteins longer than 100 amino acids, of which 37 correspond to known genes and 29 more show some similarity to sequences in databases. Of 55 new open reading frames analysed by gene disruption, three are essential genes; of 42 non-essential genes that were tested, 14 show some discernible effect on phenotype and the remaining 28 have no overt function.read more
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Journal ArticleDOI
Initial sequencing and analysis of the human genome.
Eric S. Lander,Lauren Linton,Bruce W. Birren,Chad Nusbaum,Michael C. Zody,Jennifer Baldwin,Keri Devon,Ken Dewar,Michael Doyle,William Fitzhugh,Roel Funke,Diane Gage,Katrina Harris,Andrew Heaford,John Howland,Lisa Kann,Jessica A. Lehoczky,Rosie Levine,Paul A. McEwan,Kevin McKernan,James Meldrim,Jill P. Mesirov,Cher Miranda,William Morris,Jerome Naylor,Christina Raymond,Mark Rosetti,Ralph Santos,Andrew Sheridan,Carrie Sougnez,Nicole Stange-Thomann,Nikola Stojanovic,Aravind Subramanian,Dudley Wyman,Jane Rogers,John Sulston,R Ainscough,Stephan Beck,David Bentley,John Burton,C M Clee,Nigel P. Carter,Alan Coulson,Rebecca Deadman,Panos Deloukas,Andrew Dunham,Ian Dunham,Richard Durbin,Lisa French,Darren Grafham,Simon G. Gregory,Tim Hubbard,Sean Humphray,Adrienne Hunt,Matthew Jones,Christine Lloyd,Amanda McMurray,Lucy Matthews,Simon Mercer,Sarah Milne,James C. Mullikin,Andrew J. Mungall,Robert W. Plumb,Mark T. Ross,Ratna Shownkeen,Sarah Sims,Robert H. Waterston,Richard K. Wilson,LaDeana W. Hillier,John Douglas Mcpherson,Marco A. Marra,Elaine R. Mardis,Lucinda Fulton,Asif T. Chinwalla,Kymberlie H. Pepin,Warren Gish,Stephanie L. Chissoe,Michael C. Wendl,Kim D. Delehaunty,Tracie L. Miner,Andrew Delehaunty,Jason B. Kramer,Lisa Cook,Robert S. Fulton,Douglas L. Johnson,Patrick Minx,Sandra W. Clifton,Trevor Hawkins,Elbert Branscomb,Paul Predki,Paul G. Richardson,Sarah Wenning,Tom Slezak,Norman A. Doggett,Jan Fang Cheng,Anne S. Olsen,Susan Lucas,Christopher J. Elkin,Edward Uberbacher,Marvin Frazier,Richard A. Gibbs,Donna M. Muzny,Steven E. Scherer,John Bouck,Erica Sodergren,Kim C. Worley,Catherine M. Rives,James H. Gorrell,Michael L. Metzker,Susan L. Naylor,Raju Kucherlapati,David L. Nelson,George M. Weinstock,Yoshiyuki Sakaki,Asao Fujiyama,Masahira Hattori,Tetsushi Yada,Atsushi Toyoda,Takehiko Itoh,Chiharu Kawagoe,Hidemi Watanabe,Yasushi Totoki,Todd D. Taylor,Jean Weissenbach,Roland Heilig,William Saurin,François Artiguenave,Philippe Brottier,Thomas Brüls,Eric Pelletier,Catherine Robert,Patrick Wincker,André Rosenthal,Matthias Platzer,Gerald Nyakatura,Stefan Taudien,Andreas Rump,Douglas R. Smith,Lynn Doucette-Stamm,Marc Rubenfield,Keith Weinstock,Mei Lee Hong,Joann Dubois,Huanming Yang,Jun Yu,Jian Wang,Guyang Huang,Jun Gu,Leroy Hood,Lee Rowen,Anup Madan,Shizen Qin,Ronald W. Davis,Nancy A. Federspiel,A. Pia Abola,Michael Proctor,Bruce A. Roe,Feng Chen,Huaqin Pan,Juliane Ramser,Hans Lehrach,Richard Reinhardt,W. Richard McCombie,Melissa De La Bastide,Neilay Dedhia,H. Blöcker,K. Hornischer,Gabriele Nordsiek,Richa Agarwala,L. Aravind,Jeffrey A. Bailey,Alex Bateman,Serafim Batzoglou,Ewan Birney,Peer Bork,Daniel G. Brown,Christopher B. Burge,Lorenzo Cerutti,Hsiu Chuan Chen,Deanna M. Church,Michele Clamp,Richard R. Copley,Tobias Doerks,Sean R. Eddy,Evan E. Eichler,Terrence S. Furey,James E. Galagan,James G. R. Gilbert,Cyrus L. Harmon,Yoshihide Hayashizaki,David Haussler,Henning Hermjakob,Karsten Hokamp,Wonhee Jang,L. Steven Johnson,Thomas A. Jones,Simon Kasif,Arek Kaspryzk,Scot Kennedy,W. James Kent,Paul Kitts,Eugene V. Koonin,Ian F Korf,David Kulp,Doron Lancet,Todd M. Lowe,Aoife McLysaght,Tarjei S. Mikkelsen,John V. Moran,Nicola Mulder,Victor J. Pollara,Chris P. Ponting,Greg Schuler,Jörg Schultz,Guy Slater,Arian F.A. Smit,Elia Stupka,Joseph Szustakowki,Danielle Thierry-Mieg,Jean Thierry-Mieg,Lukas Wagner,John W. Wallis,Raymond Wheeler,Alan Williams,Yuri I. Wolf,Kenneth H. Wolfe,Shiaw Pyng Yang,Ru Fang Yeh,Francis S. Collins,Mark S. Guyer,Jane Peterson,Adam Felsenfeld,Kris A. Wetterstrand,Richard M. Myers,Jeremy Schmutz,Mark Dickson,Jane Grimwood,David R. Cox,Maynard V. Olson,Rajinder Kaul,Christopher K. Raymond,Nobuyoshi Shimizu,Kazuhiko Kawasaki,Shinsei Minoshima,Glen A. Evans,Maria Athanasiou,Roger A. Schultz,Aristides Patrinos,Michael J. Morgan +248 more
TL;DR: The results of an international collaboration to produce and make freely available a draft sequence of the human genome are reported and an initial analysis is presented, describing some of the insights that can be gleaned from the sequence.
Journal ArticleDOI
Life with 6000 Genes
André Goffeau,Bart Barrell,Howard Bussey,Ronald W. Davis,Bernard Dujon,Horst Feldmann,Francis Galibert,J D Hoheisel,Claude Jacq,Mark Johnston,Edward J. Louis,Hans-Werner Mewes,Yasufumi Murakami,Peter Philippsen,Hervé Tettelin,Stephen G. Oliver +15 more
TL;DR: The genome of the yeast Saccharomyces cerevisiae has been completely sequenced through a worldwide collaboration and provides information about the higher order organization of yeast's 16 chromosomes and allows some insight into their evolutionary history.
Journal ArticleDOI
Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications.
Carrie Baker Brachmann,Adrian Davies,Gregory J. Cost,Emerita Caputo,Joachim J. Li,Joachim J. Li,Philip Hieter,Jef D. Boeke +7 more
TL;DR: A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed and will reduce plasmid integration events which can interfere with a wide variety of molecular genetic applications.
Journal ArticleDOI
New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiae
TL;DR: A dominant resistance module, for selection of S. cerevisiae transformants, which entirely consists of heterologous DNA is constructed and tested, and some kanMX modules are flanked by 470 bp direct repeats, promoting in vivo excision with frequencies of 10–3–10–4.
Journal ArticleDOI
Genomic Libraries and a Host Strain Designed for Highly Efficient Two-Hybrid Selection in Yeast
TL;DR: A novel multienzyme approach was used to generate a set of highly representative genomic libraries from S. cerevisiae and a unique host strain was created that contains three easily assayed reporter genes, each under the control of a different inducible promoter.
References
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