Sao Paulo State University

About: Sao Paulo State University is a education organization based out in São Paulo, Brazil. It is known for research contribution in the topics: Population & Context (language use). The organization has 55715 authors who have published 100436 publications receiving 1375332 citations.

Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyte

Abstract: Due to the exclusively maternal inheritance of mitochondria, mitochondrial genotypes can be coupled to a particular nuclear genotype by continuous mating of founder females and their female offspring to males of the desired nuclear genotype. However, backcrossing is a gradual procedure that, apart from being lengthy, cannot ascertain that genetic and epigenetic changes will modify the original nuclear genotype. Animal cloning by nuclear transfer using host ooplasm carrying polymorphic mitochondrial genomes allows, among other biotechnology applications, the coupling of nuclear and mitochondrial genotypes of diverse origin within a single generation. Previous attempts to use Bos taurus oocytes as hosts to transfer nuclei from unrelated species led to the development to the blastocyst stage but none supported gestation to term. Our aim in this study was to determine whether B. taurus oocytes support development of nuclei from the closely related B. indicus cattle and to examine the fate of their mitochondrial genotypes throughout development. We show that indicus:taurus reconstructed oocytes develop to the blastocyst stage and produce live offspring after transfer to surrogate cows. We also demonstrate that, in reconstructed embryos, donor cell-derived mitochondria undergo a stringent genetic drift during early development leading, in most cases, to a reduction or complete elimination of B. indicus mtDNA. These results demonstrate that cross-subspecies animal cloning is a viable approach both for matching diverse nuclear and cytoplasmic genes to create novel breeds of cattle and for rescuing closely related endangered cattle.

Effect of low-level laser therapy on bone repair: Histological study in rats

Abstract: UNESP, Fac Odontol Araraquara, Dept Orthodont & Pediat Dent, Sch Dent Araraquara, BR-14801903 Araraquara, SP, Brazil

Transmissible Dog Cancer Genome Reveals the Origin and History of an Ancient Cell Lineage

Abstract: Canine transmissible venereal tumor (CTVT) is the oldest known somatic cell lineage. It is a transmissible cancer that propagates naturally in dogs. We sequenced the genomes of two CTVT tumors and found that CTVT has acquired 1.9 million somatic substitution mutations and bears evidence of exposure to ultraviolet light. CTVT is remarkably stable and lacks subclonal heterogeneity despite thousands of rearrangements, copy-number changes, and retrotransposon insertions. More than 10,000 genes carry nonsynonymous variants, and 646 genes have been lost. CTVT first arose in a dog with low genomic heterozygosity that may have lived about 11,000 years ago. The cancer spawned by this individual dispersed across continents about 500 years ago. Our results provide a genetic identikit of an ancient dog and demonstrate the robustness of mammalian somatic cells to survive for millennia despite a massive mutation burden.

Search for long-lived particles that decay into final states containing two electrons or two muons in proton-proton collisions at s =8TeV

Abstract: A search is performed for long-lived particles that decay into final states that include a pair of electrons or a pair of muons. The experimental signature is a distinctive topology consisting of a pair of charged leptons originating from a displaced secondary vertex. Events corresponding to an integrated luminosity of 19.6 (20.5) inverse femtobarns in the electron (muon) channel were collected with the CMS detector at the CERN LHC in proton-proton collisions at sqrt(s) = 8 TeV. No significant excess is observed above standard model expectations. Upper limits on the product of the cross section and branching fraction of such a signal are presented as a function of the long-lived particle's mean proper decay length. The limits are presented in an approximately model-independent way, allowing them to be applied to a wide class of models yielding the above topology. Over much of the investigated parameter space, the limits obtained are the most stringent to date. In the specific case of a model in which a Higgs boson in the mass range 125-1000 GeV decays into a pair of long-lived neutral bosons in the mass range 20-350 GeV, each of which can then decay to dileptons, the upper limits obtained are typically in the range 0.2-10 fb for mean proper decay lengths of the long-lived particles in the range 0.01-100 cm. In the case of the lowest Higgs mass considered (125 GeV), the limits are in the range 2-50 fb. These limits are sensitive to Higgs boson branching fractions as low as 1E-4.

Wire and arc additive manufacturing of HSLA steel: Effect of thermal cycles on microstructure and mechanical properties

Abstract: Wire and arc additive manufacturing (WAAM) is a viable technique for the manufacture of large and complex dedicated parts used in structural applications. High-strength low-alloy (HSLA) steels are well-known for their applications in the tool and die industries and as power-plant components. The microstructure and mechanical properties of the as-built parts are investigated, and are correlated with the thermal cycles involved in the process. The heat input is found to affect the cooling rates, interlayer temperatures, and residence times in the 800–500 °C interval when measured using an infrared camera. The microstructural characterization performed by scanning electron microscopy reveals that the microstructural constituents of the sample remain unchanged. i.e., the same microstructural constituents—ferrite, bainite, martensite, and retained austenite are present for all heat inputs. Electron backscattered diffraction analysis shows that no preferential texture has been developed in the samples. Because of the homogeneity in the microstructural features of the as-built parts, the mechanical properties of the as-built parts are found to be nearly isotropic. Mechanical testing of samples shows excellent ductility and high mechanical strength. This is the first study elucidating on the effect of thermal cycles on the microstructure and mechanical properties during WAAM of HSLA steel.