Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
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TLDR
This is the first complete genome sequence of a plant and provides the foundations for more comprehensive comparison of conserved processes in all eukaryotes, identifying a wide range of plant-specific gene functions and establishing rapid systematic ways to identify genes for crop improvement.Abstract:
The flowering plant Arabidopsis thaliana is an important model system for identifying genes and determining their functions. Here we report the analysis of the genomic sequence of Arabidopsis. The sequenced regions cover 115.4 megabases of the 125-megabase genome and extend into centromeric regions. The evolution of Arabidopsis involved a whole-genome duplication, followed by subsequent gene loss and extensive local gene duplications, giving rise to a dynamic genome enriched by lateral gene transfer from a cyanobacterial-like ancestor of the plastid. The genome contains 25,498 genes encoding proteins from 11,000 families, similar to the functional diversity of Drosophila and Caenorhabditis elegans--the other sequenced multicellular eukaryotes. Arabidopsis has many families of new proteins but also lacks several common protein families, indicating that the sets of common proteins have undergone differential expansion and contraction in the three multicellular eukaryotes. This is the first complete genome sequence of a plant and provides the foundations for more comprehensive comparison of conserved processes in all eukaryotes, identifying a wide range of plant-specific gene functions and establishing rapid systematic ways to identify genes for crop improvement.read more
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Differential targeting of GSH1 and GSH2 is achieved by multiple transcription initiation: implications for the compartmentation of glutathione biosynthesis in the Brassicaceae.
TL;DR: A detailed analysis of the 5'ends of GSH1 and GSH2 mRNAs is reported and the subcellular targeting of the proteins encoded by different transcript types is demonstrated, demonstrating an exclusive targeting of G SH1 to the plastids.
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TL;DR: A subcellular proteomic approach to discover new envelope transporters was developed, which combined the use of highly purified and characterized membrane fractions, extraction of the hydrophobic proteins with organic solvents, SDS/PAGE separation, and tandem mass spectrometry analysis.
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A new method for rapid visualization of defects in leaf cuticle reveals five intrinsic patterns of surface defects in Arabidopsis
TL;DR: The TB test is demonstrated to be a rapid and inexpensive method for detection of cuticular defects in whole leaves of mutants of Arabidopsis thaliana, including abnormal leaf shape1, fiddlehead, and five eceriferum mutants, in which the structure and/or function of the cuticle is abnormal.
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The Arabidopsis nitrate transporter NRT2.5 plays a role in nitrate acquisition and remobilization in nitrogen‐starved plants
Lina Lezhneva,Lina Lezhneva,Takatoshi Kiba,Ana-Belen Feria-Bourrellier,Ana-Belen Feria-Bourrellier,Florence Lafouge,Florence Lafouge,Stéphanie Boutet-Mercey,Stéphanie Boutet-Mercey,Parzhak Zoufan,Parzhak Zoufan,Hitoshi Sakakibara,Françoise Daniel-Vedele,Françoise Daniel-Vedele,Anne Krapp,Anne Krapp +15 more
TL;DR: Arabidopsis NITRATE TRANSPORTER 2.5 (NRT2.5) is a plasma membrane-localized high-affinity nitrate transporter playing an essential role in adult plants under severe nitrogen starvation and becomes the most abundant transcript amongst the seven NRT2 family members in shoots and roots of adult plants after long-term starvation.
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Structural relatedness of plant food allergens with specific reference to cross-reactive allergens: An in silico analysis
TL;DR: Structural bioinformatic analysis of conserved exterior main chains and amino acid side chains in cross-reactive homologues of Bet v 1 and nonspecific lipid transfer proteins showed higher levels of similarity than shown by simple sequence comparisons.
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TL;DR: The nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome is determined using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map.
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