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Open AccessJournal ArticleDOI

Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.

Arabidopsis Genome Initiative
- 14 Dec 2000 - 
- Vol. 408, Iss: 6814, pp 796-815
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TLDR
This is the first complete genome sequence of a plant and provides the foundations for more comprehensive comparison of conserved processes in all eukaryotes, identifying a wide range of plant-specific gene functions and establishing rapid systematic ways to identify genes for crop improvement.
Abstract
The flowering plant Arabidopsis thaliana is an important model system for identifying genes and determining their functions. Here we report the analysis of the genomic sequence of Arabidopsis. The sequenced regions cover 115.4 megabases of the 125-megabase genome and extend into centromeric regions. The evolution of Arabidopsis involved a whole-genome duplication, followed by subsequent gene loss and extensive local gene duplications, giving rise to a dynamic genome enriched by lateral gene transfer from a cyanobacterial-like ancestor of the plastid. The genome contains 25,498 genes encoding proteins from 11,000 families, similar to the functional diversity of Drosophila and Caenorhabditis elegans--the other sequenced multicellular eukaryotes. Arabidopsis has many families of new proteins but also lacks several common protein families, indicating that the sets of common proteins have undergone differential expansion and contraction in the three multicellular eukaryotes. This is the first complete genome sequence of a plant and provides the foundations for more comprehensive comparison of conserved processes in all eukaryotes, identifying a wide range of plant-specific gene functions and establishing rapid systematic ways to identify genes for crop improvement.

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Journal ArticleDOI

Identification and characterization of new plant microRNAs using EST analysis

TL;DR: The findings suggest that EST analysis is a good alternative strategy for identifying new miRNA candidates, their targets, and other genes.
Journal ArticleDOI

A Link between ethylene and auxin uncovered by the characterization of two root-specific ethylene-insensitive mutants in Arabidopsis.

TL;DR: It is shown that ethylene-triggered inhibition of root growth, one of the classical effects of ethylene in Arabidopsis thaliana seedlings, is mediated by the action of the WEAK ETHYLENE INSENSITIVE2/ANTHRANILATE SYNTHASE α1 and ASA1 genes that encode α- and β-subunits of a rate-limiting enzyme of Trp biosynthesis, anthranilate synthase.
Journal ArticleDOI

Overexpression of a zinc finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco

TL;DR: The isolation and characterization of an intronless gene (OSISAP1) from rice encoding a zinc-finger protein that is induced after different types of stresses, namely cold, desiccation, salt, submergence, and heavy metals as well as injury is described.
Journal ArticleDOI

Molecular Characterization of Functional Domains in the Protein Kinase SOS2 That Is Required for Plant Salt Tolerance

TL;DR: The identification of a SOS3 binding motif in SOS2 that also serves as the kinase autoinhibitory domain is reported, providing insights into regulation of the Kinase activities of SOS2 and the SOS2 family of protein kinases.
References
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Journal ArticleDOI

Basic Local Alignment Search Tool

TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI

tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.

TL;DR: A program is described, tRNAscan-SE, which identifies 99-100% of transfer RNA genes in DNA sequence while giving less than one false positive per 15 gigabases.
Journal ArticleDOI

The Complete Genome Sequence of Escherichia coli K-12

TL;DR: The 4,639,221-base pair sequence of Escherichia coli K-12 is presented and reveals ubiquitous as well as narrowly distributed gene families; many families of similar genes within E. coli are also evident.
Journal ArticleDOI

SCOP: a structural classification of proteins database for the investigation of sequences and structures.

TL;DR: This database provides a detailed and comprehensive description of the structural and evolutionary relationships of the proteins of known structure and provides for each entry links to co-ordinates, images of the structure, interactive viewers, sequence data and literature references.
Journal ArticleDOI

The genome sequence of Drosophila melanogaster

Mark Raymond Adams, +194 more
- 24 Mar 2000 - 
TL;DR: The nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome is determined using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map.
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