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Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
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TLDR
Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
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Epigenetics as a therapeutic target in breast cancer.
Roisin M. Connolly,Vered Stearns +1 more
TL;DR: The critical role of epigenetics in breast cancer is reviewed including the potential for epigenetic alterations to serve as biomarkers determining breast cancer prognosis and response to therapy and initial promising results to date are highlighted.
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Advances in DNA methylation: 5-hydroxymethylcytosine revisited.
TL;DR: The formation of 5hmC can lead to demethylation of DNA, which may contribute to the dynamics of DNA methylation, and future challenges include better understanding the normal molecular, cellular and physiological roles of 5mC and TET proteins.
Journal ArticleDOI
5-azacytidine induces cardiac differentiation of P19 embryonic stem cells
TL;DR: It is demonstrated that 5-aza induces differentiation of P19 cells into cardiomyocytes in a confluent monolayer culture in the absence of prior embryoid formation and dimethyl sulfoxide exposure, depending in part on alteration of BMP signaling molecules.
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DNA methylation patterns in lung carcinomas
Gerd P. Pfeifer,Tibor A. Rauch +1 more
TL;DR: Using a genome-scale mapping technique for CpG methylation (MIRA-chip), DNA hypomethylation at CpGs in squamous cell tumors preferentially affects repetitive sequence classes including SINEs, LINEs, subtelomeric repeats, and segmental duplications.
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Donor cells for nuclear cloning: many are called, but few are chosen.
Björn Oback,David N. Wells +1 more
TL;DR: This review summarizes current theoretical aspects of donor cell selection and focuses on the impact of genetic and epigenetic differences between donor cell types on successful mammalian cloning.
References
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Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
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DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
Journal ArticleDOI
X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
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Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.