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Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
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TLDR
Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
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Mutation at autosomal loci of Chinese hamster ovary cells: involvement of a high-frequency event silencing two linked alleles.
TL;DR: A model is proposed, similar to that for the mutational events at the adenine phosphoribosyl transferase locus, of two different events, high and low frequency, being responsible for mutation at either of the linked loci tk and glk, and of all class I glk+/- lines examined were functionally hemizygous at tk.
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Enhanced in vitro reactivation of herpes simplex virus type 2 from latently infected guinea-pig neural tissues by 5-azacytidine.
TL;DR: Treatment of explant cultures of latently herpes simplex virus type 2 (HSV-2)-infected guinea-pig dorsal root ganglia and spinal cords in vitro with 5-AZC significantly enhanced the rate of HSV recovery, supporting a role for methylation in HSV latency and reactivation.
Journal ArticleDOI
Effects of complex extracellular matrices on 5-azacytidine-induced myogenesis.
TL;DR: Analysis of the matrix glycosaminoglycans showed that high chondroitin sulfate relative to hyaluronic acid levels were favorable for the myogenic response and had a major influence on mesenchymal differentiation.
Journal ArticleDOI
Blood glutathione redox status and global methylation of peripheral blood mononuclear cell DNA in Bangladeshi adults.
Megan M. Niedzwiecki,Megan N. Hall,Xinhua Liu,Julie Oka,Kristin N. Harper,Vesna Slavkovich,Vesna Ilievski,Diane Levy,Alexander van Geen,Jacob L. Mey,Shafiul Alam,Abu B. Siddique,Faruque Parvez,Joseph H. Graziano,Mary V. Gamble +14 more
TL;DR: The hypothesis that a more oxidized blood GSH redox status is associated with decreased global methylation of PBMC DNA is supported and mechanisms through which cellular redox might influence global DNA methylation should be explored.
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Epigenetic regulation of immune checkpoints: another target for cancer immunotherapy?
Mahmoud A. Ali,Marwa Matboli,Marwa Tarek,Maged Reda,Kamal M Kamal,Mahmoud Nouh,Ahmed M Ashry,Ahmed Fath El-Bab,Hend A Mesalam,Ayman El-Sayed Shafei,Omar Abdel-Rahman +10 more
TL;DR: How the potential interaction between epigenetic and immune modulation may lead to new approaches for specific epigenome/immunome-targeted therapies for cancer is shown.
References
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A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
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DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
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X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
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Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.