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Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
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TLDR
Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
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Decitabine Dosing Schedules
TL;DR: The drug is less active and more toxic when administered via continuous infusion, and increasing dose-intensity resulted in a higher response rate, suggesting a relationship between peak drug levels and response.
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The mat-1 gene in chlamydomonas regulates DNA methylation during gametogenesis
TL;DR: The distribution of 5- methyl cytosine residues in restriction fragments of chloroplast DNA sampled during gametogenesis is described by two methods: ethidium bromide staining of agarose gels, and binding of antibody directed against 5-methyl cytosines onto restriction fragments blotted to nitro-cellulose paper.
Journal ArticleDOI
A combination treatment with DNA methyltransferase inhibitors and suramin decreases invasiveness of breast cancer cells
TL;DR: It is shown that when used in combination, suramin and DMTIs impair the invasive phenotype of breast cancer cells, and PKD1, a kinase that previously has been described as a suppressor of tumor cell invasion, is an interface for both FDA-approved drugs.
Journal ArticleDOI
Cytotoxicity of 5-aza-2'-deoxycytidine in a mammalian cell system.
TL;DR: After the addition of 5-aza-2'-deoxycytidine, a potent inhibitor of DNA methylation and S-phase-specific cytotoxic agent, metaphase chromosomes of Chinese hamster ovary cells exhibited a highly decondensed and extended morphology at the first mitotic division.
Journal ArticleDOI
DNA methylation prevents transfection of genes for specific surface antigens.
TL;DR: Investigation of the transfectability of HLA class I genes and of the genes for the T-cell differentiation antigens Leu-1 (CD5) andLeu-2 (CD8) in mouse L cells by using human sperm cells and choriocarcinoma cell lines, tumors of trophoblastic origin, indicates that DNA methylation affects the efficiency of transfection of surface antigen genes in L cells.
References
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Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
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X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
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Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.