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Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
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TLDR
Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
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MSX1 inhibits MyoD expression in fibroblast × 10T½ cell hybrids
Paul I. Woloshin,Kening Song,Kening Song,Catherine Degnin,Ann Mc Neill Killary,David J. Goldhamer,David Sassoon,Mathew J. Thayer +7 more
TL;DR: It is concluded that MSX1 inhibits transcription of myoD and that myo D is a target for homeobox gene regulation.
Journal ArticleDOI
Epigenetic Regulation of Human Trophoblastic Cell Migration and Invasion
TL;DR: Epigenetic regulation of plakoglobin and E-cadherin is pivotal for appropriate trophoblastic invasion in vitro and is examined by use of the choriocarcinoma cell line, BeWo.
Journal ArticleDOI
Epigenetic drug discovery: targeting DNA methyltransferases.
Jason M. Foulks,K. Mark Parnell,Rebecca N. Nix,Suzanna Chau,K. Swierczek,Michael Saunders,Kevin Wright,Hendrickson Thomas,Koc-Kan Ho,Michael V. McCullar,Steven B. Kanner +10 more
TL;DR: This review focuses on the rationale of targeting DNMTs in cancer, the historical approach to DNMT inhibition, and current marketed hypomethylating therapeutics azacytidine and decitabine, and novel DNMT inhibitory agents emerging in development.
Journal ArticleDOI
Limited gene activation in tumor and normal epithelial cells treated with the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine.
TL;DR: It is proposed that 5-aza-CdR-mediated gene activation has two distinct requirements: 1) the reversal of promoter hypermethylation, and 2) the presence of transcriptional activators competent for the activation of hypomethylated target promoters.
Journal ArticleDOI
Effect of an inhibitor of DNA methylation on T cells. II. 5-azacytidine induces self-reactivity in antigen-specific T4+ cells
TL;DR: Cloned, T4+ Interleukin-2 dependent, antigen-specific T cells were treated with an inhibitor of DNA methylation, 5-azacytidine (5-azaC), and lost the requirement for antigen and could be activated by autologous macrophages alone.
References
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A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
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DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
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X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
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Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.