Journal ArticleDOI
Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
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TLDR
Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
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Journal ArticleDOI
5-Aza-2′-deoxycytidine induces growth inhibition and upregulation of epidermal growth factor receptor on human epithelial cancer cells
Michele Caraglia,Antonio Pinto,Pierpaolo Correale,Vittorina Zagonel,Geppino Genua,A Leardi,Stefano Pepe,Ar Bianco,Pierosandro Tagliaferri +8 more
TL;DR: A role is suggested for 5azaCdR in enhancing the efficacy of therapeutic approaches involving the use of anti-EGF-R immunoconjugated for the imaging and the treatment of human epithelial neoplasias.
Journal ArticleDOI
Muscle tissue engineering and regeneration through epigenetic reprogramming and scaffold manipulation.
TL;DR: The experiments demonstrated that cellular plasticity and trans-differentiation were significantly enhanced when ADSCs were treated with an effective dose of 5-Aza-CR and suggest that both physical signals and chemical milieu are critical for the regulation of cellular responses.
Journal ArticleDOI
DNA-dependent protein kinase (DNA-PK)-deficient human glioblastoma cells are preferentially sensitized by Zebularine
TL;DR: It is reported that the DNA-demethylating agent Zebularine preferentially sensitizes the killing of human glioblastomas deficient in DNA-dependent protein kinase (DNA-PK), and suggests that DNA-PK is the major determining factor for cellular response to ZebULARine.
Book ChapterDOI
Complexities in Gene Regulation by Promoter Methylation
TL;DR: The regulation of eukaryotic gene expression presents a multi-faceted problem that implies the facility of gene activity to be modulated to various degrees and of promoters to be capable of responding to a variety of internal and environmental stimuli.
Journal ArticleDOI
DNMTs and Impact of CpG Content, Transcription Factors, Consensus Motifs, lncRNAs, and Histone Marks on DNA Methylation.
TL;DR: This work focuses special attention on the DNMTs’ specificity for target sites, including key cis-regulatory factors such as CpG content, common motifs, transcription factors binding sites, lncRNAs, and histone marks to regulate DNA methylation.
References
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A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
Journal ArticleDOI
X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
Journal ArticleDOI
Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.