Journal ArticleDOI
Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
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TLDR
Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
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Ubiquitous and tenacious methylation of the CpG site in codon 248 of the p53 gene may explain its frequent appearance as a mutational hot spot in human cancer.
A N Magewu,Peter A. Jones +1 more
TL;DR: The frequent methylation of codon 248 and adjacent Alu sequence may explain the enhanced mutability of this site as a result of the deamination of the 5-methylcytosine.
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Studies of X chromosome DNA methylation in normal human cells
TL;DR: No derepression of the inactive X is observed in clonal populations of normal human fibroblasts treated with 5-azacytidine, which preclude ubiquitous methylation differences as the molecular basis for X-chromosome inactivation.
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Unusual methylation pattern of the α2(I) collagen gene
TL;DR: The data indicate that a gene that is methylated can be actively transcribed and that the level of expression of the α2 (type I) collagen gene seems to be independent of methylation.
Journal ArticleDOI
Dual targeting of epigenetic therapy in cancer
TL;DR: The therapeutic potential of DNMT and HDAC inhibitors as anti-cancer drugs, as evaluated in clinical trials, and their angiostatic activities, apart from their inhibitory effects on tumor cells are reviewed.
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Growth and differentiation of rat bone marrow stromal cells: does 5-azacytidine trigger their cardiomyogenic differentiation?
TL;DR: Rat MSCs cannot be extensively expanded in vitro or be induced to differentiate in an expected cardiomyogenic way by 5-azacytidine-treatment, if the cells are not immortalized.
References
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Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
Journal ArticleDOI
X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
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Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.