Journal ArticleDOI
Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
Reads0
Chats0
TLDR
Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
Citations
More filters
Journal ArticleDOI
Comparison of antineoplastic activity of cytosine arabinoside and 5-aza-2'-deoxycytidine against human leukemic cells of different phenotype.
TL;DR: There are significant differences in the antineoplastic activity of Ara-C and 5-AZA-CdR against human leukemic cell lines of different phenotype and that these differences are related to Differences in the metabolism of these two deoxycytidine analogues and to their effects on DNA synthesis and methylation.
Journal ArticleDOI
Alterations in the flow of one-carbon units affect KinB-dependent sporulation in Bacillus subtilis.
TL;DR: The results indicate that lrpA–lrpB together with the biosynthetic glyA gene lie on a common signalling pathway meant to activate the KinB sensor kinase.
Journal ArticleDOI
5-Azacytidine treatment of the fission yeast leads to cytotoxicity and cell cycle arrest
TL;DR: It is demonstrated that 5-azaC treatment of Schizosaccharomyces pombe leads to reversible cell cycle arrest at the G2/M transition, and the data show that viability in the presence of the drug requires both the DNA damage and the replication checkpoint pathways to be functional.
Book ChapterDOI
Hormonal control of adipocyte differentiation and adipocyte gene expression.
TL;DR: This chapter presents a summarization of a large number of studies aimed at elucidating the signaling system by which determined cells decide to activate a terminal differentiation program.
Journal ArticleDOI
5-azacytidine Induction of thymidine kinase in a spontaneously enzyme-deficient murine tumor line.
TL;DR: The ability of 5-azacytidine to induce TK activity in a spontaneously enzyme-deficient murine tumor cell line is demonstrated and suggested that the TK deficiency in the L61-M cell line was due in part to an alteration in the methylation pattern of DNA, resulting in the diminished expression of the Tk gene.
References
More filters
Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
Journal ArticleDOI
X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
Journal ArticleDOI
Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.