Journal ArticleDOI
Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
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Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
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DNA methylation aberrancies as a guide for surveillance and treatment of human cancers.
TL;DR: The integration of DNA methylation inhibitors with conventional chemotherapies, DNA repair inhibitors and immune-based therapies are described to bring the epigenome closer to its normal state and increase sensitivity to other therapeutic agents to improve patient outcome and survival.
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Chromatin, cancer and drug therapies
Connie C. Cortez,Peter A. Jones +1 more
TL;DR: The structure and organization of chromatin have attracted a great deal of attention recently because of their implications for the field of epigenetics and progress has been made in the treatment of hematological malignancies and some solid tumors.
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5-azacytidine-induced re-expression of alleles on the inactive X chromosome in a hybrid mouse cell line.
TL;DR: Re-expression of the Hpt and Gpd loci appears to be co-ordinated to some extent; however, no HPRT+ reactivants, nor unselected colonies re-expressed M. caroli PGK-A, and the hypothesis that DNA methylation is involved in mammalian X chromosome inactivation is tested.
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Epigenetic-based therapies in cancer: progress to date.
TL;DR: Current advances in epigenetic therapies are reviewed with a focus on recently reported clinical trials, and hypomethylating agents and HDAC inhibitors seem to be promising for treating several types of cancer.
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10T1/2 cells: an in vitro model for molecular genetic analysis of mesodermal determination and differentiation.
TL;DR: The most significant discovery has been that 10T1/2 lineage determination is under simple genetic control and that the regulatory genes that mediate the formation of myogenic cell lineages, and likely the chondrogenic and adipogenic lineages can be demonstrated and studied by genomic DNA and cDNA transfection approaches.
References
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Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
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DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
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X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
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Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.