Journal ArticleDOI
Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
Reads0
Chats0
TLDR
Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
Citations
More filters
Journal ArticleDOI
Region-specific methylation of the parathyroid hormone-related peptide gene determines its expression in human renal carcinoma cell lines.
TL;DR: Using a system of 12 human renal carcinoma cell lines, it was found that the expression of the PTHrP gene in these cell lines is controlled at the transcriptional level, and the functional importance of this mechanism of control was confirmed by the ability of the demethylating agent, 5-azacytidine, to induce P THrP mRNA expression in previously nonexpressing cell lines.
Journal ArticleDOI
6-Thioguanine perturbs cytosine methylation at the CpG dinucleotide site by DNA methyltransferases in vitro and acts as a DNA demethylating agent in vivo.
Hongxia Wang,Yinsheng Wang +1 more
TL;DR: It is demonstrated that the treatment of Jurkat T cells, which were derived from acute lymphoblastic leukemia, with 6-thioguanine could result in an appreciable drop in the level of global cytosine methylation, and showed that 6-THiOGuanine, after being incorporated into DNA, may perturb the epigenetic pathway of gene regulation.
Journal ArticleDOI
Molecular regulation of CC-chemokine receptor 3 expression in human T helper 2 cells.
TL;DR: In Th2 cells both cytokine production and migratory capacity are regulated through a similar mechanism involving chromatin remodeling, and drugs that affect the chromatin structure facilitate CCR3 expression in T cells.
Journal ArticleDOI
Aberrant methylation of gene associated CpG sites occurs in borderline personality disorder.
Stefanie Teschler,Marek Bartkuhn,Natascha Künzel,Christian Schmidt,Steffen Kiehl,Gerhard Dammann,Reinhard Dammann +6 more
TL;DR: In this article, the authors performed genome wide methylation analysis and revealed specific CpG sites that exhibited increased methylation in 24 female BPD patients compared to 11 female healthy controls.
Journal ArticleDOI
Recurrent Loss of Specific Introns during Angiosperm Evolution
TL;DR: This study provides the first comprehensive analysis of recurrent intron loss, makes a series of novel findings on the patterns of recurrentintron loss during the evolution of the grass family, and provides insight into the molecular mechanism(s) underlying introns loss.
References
More filters
Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
Journal ArticleDOI
X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
Journal ArticleDOI
Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.