Journal ArticleDOI
Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
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TLDR
Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
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Trapped in action: direct visualization of DNA methyltransferase activity in living cells
Lothar Schermelleh,Fabio Spada,Hariharan Easwaran,Kourosh Zolghadr,Jean B Margot,M. Cristina Cardoso,Heinrich Leonhardt,Heinrich Leonhardt +7 more
TL;DR: This trapping assay monitors kinetic properties and activity-dependent immobilization of DNA methyltransferases in their native environment, and makes it possible to directly compare mutations and inhibitors that affect regulation and catalytic activity ofDNA methyltransferase 1 (Dnmt1) in single living cells.
Journal ArticleDOI
Changes in phenotypic expression in embryonic and adult cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: 5‐azacytidine induced the formation of biochemically differentiated myotubes, adipocytes, and chondrocytes in the mouse embryo cell line and the induction of the muscle phenotype was cell cycle specific, while the adipocyte phenotype is also induced maximally in cells treated during early S phase.
Journal ArticleDOI
Epimutation of repeated genes in Ascobolus immersus.
TL;DR: Data show the epimutational nature of this methylation induced premeiotically (MIP) process, in the absence of selection, gene silencing and methylation were faithfully maintained through mitotic divisions and through five successive sexual cycles.
Journal ArticleDOI
Epigenetic Mechanisms and Gene Networks in the Nervous System
Christine M. Colvis,Jonathan D. Pollock,Richard H. Goodman,Soren Impey,John J. Dunn,Gail Mandel,Frances A. Champagne,Mark Mayford,Edward Korzus,Arvind Kumar,William Renthal,David E. H. Theobald,Eric J. Nestler +12 more
TL;DR: Adaptation to the environment is one of the fundamental regulatory processes in biology and is found among both simple and complex organisms.
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Evaluation of a 7-Day Continuous Intravenous Infusion of Decitabine: Inhibition of Promoter-Specific and Global Genomic DNA Methylation
Wolfram E. Samlowski,Sancy A. Leachman,Mark Wade,Pamela B. Cassidy,Patricia Porter-Gill,Leslie T. Busby,Richard H. Wheeler,Kenneth M. Boucher,Frank A. Fitzpatrick,David A. Jones,Adam R. Karpf +10 more
TL;DR: A 168-hour continuous infusion of decitabine is well tolerated and results in the inhibition of promoter-specific and genomic DNA methylation in vivo.
References
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A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
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DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
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X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
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Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.