Journal ArticleDOI
Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
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TLDR
Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
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β-adrenergic receptor induction in HeLa cells: Synergistic effect of 5-azacytidine and butyrate
Saleem Jahangeer,Saleem Jahangeer,Richard M. Elliott,Richard M. Elliott,R.C. Henneberry,R.C. Henneberry +5 more
TL;DR: Preliminary results are reported showing that β-adrenergic receptors can be induced to comparable levels by the nucleoside analogue 5-azacytidine, implying that induction of β- adrenergic receptor induction in HeLa by butyrate and5-azACYtidine may involve separate mechanisms.
Journal ArticleDOI
Effect of 5-aza-2'-deoxycytidine and retinoic acid on differentiation and c-myc expression in HL-60 myeloid leukemic cells.
TL;DR: Data indicate that 5-AZA-CdR and retinoic acid in combination produce an additive antineoplastic effect against HL-60 human myeloid leukemic cells.
Journal ArticleDOI
Report of a phase 1/2 study of a combination of azacitidine and cytarabine in acute myelogenous leukemia and high-risk myelodysplastic syndromes
Gautam Borthakur,Xuelin Huang,Hagop M. Kantarjian,Stefan Faderl,Farhad Ravandi,Alessandra Ferrajoli,Ritva Torma,Gail Morris,Donald A. Berry,Jean Pierre J. Issa +9 more
TL;DR: It is concluded that the combination of azacitidine and cytarabine is feasible but has limited activity in relapsed/refractory AML.
Journal ArticleDOI
Oral decitabine reactivates expression of the methylated γ-globin gene in Papio anubis
Donald Lavelle,Donald Lavelle,Janet Chin,Janet Chin,Kestis Vaitkus,Kestis Vaitkus,Sanjeev Redkar,Pasit Phiasivongsa,Chunlin Tang,Roselle Will,Maria Hankewych,Maria Hankewych,Bryan P. Roxas,Bryan P. Roxas,Mahipal Singh,Mahipal Singh,Yogen Saunthararajah,Joseph DeSimone,Joseph DeSimone +18 more
TL;DR: The results demonstrate that oral administration of these drugs at doses 17–34 times optimal subcutaneous doses of decitabine reactivates fetal hemoglobin, demethylates the ϵ‐ and γ‐globin gene promoters, and increases histone acetylation of these promoters in baboons (Papio anubis).
Journal ArticleDOI
Safety and clinical activity of 5-aza-2'-deoxycytidine (decitabine) with or without Hyper-CVAD in relapsed/refractory acute lymphocytic leukaemia.
Christopher B. Benton,Deborah A. Thomas,Hui Yang,Farhad Ravandi,Michael Rytting,Susan O'Brien,Anna Franklin,Gautam Borthakur,Samuel Dara,Monica Kwari,Sherry Pierce,Elias Jabbour,Hagop M. Kantarjian,Guillermo Garcia-Manero +13 more
TL;DR: Decitabine alone or given with Hyper‐CVAD is safe and has clinical activity in patients with advanced ALL and grade 3 or 4 toxic effects included non‐life‐threatening hepatotoxicity and hyperglycaemia.
References
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A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
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DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
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X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
Journal ArticleDOI
Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.