Journal ArticleDOI
Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
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TLDR
Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
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Epigenetic Down-Regulation and Suppressive Role of DCBLD2 in Gastric Cancer Cell Proliferation and Invasion
Mirang Kim,Kyung Tae Lee,Hay-Ran Jang,Jeong-Hwan Kim,Seung-Moo Noh,Kyu-Sang Song,June-Sik Cho,Hyun Yong Jeong,Seon-Young Kim,Hyang-Sook Yoo,Yong Sung Kim +10 more
TL;DR: Down-regulation of DCBLD2, often associated with promoter hypermethylation, is a frequent event that may be related to the development of gastric cancer.
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A phase I, pharmacokinetic, and pharmacodynamic evaluation of the DNA methyltransferase inhibitor 5-fluoro-2'-deoxycytidine, administered with tetrahydrouridine.
Edward M. Newman,Robert J. Morgan,Shivaani Kummar,Jan H. Beumer,M. Suzette Blanchard,Christopher Ruel,Anthony B. El-Khoueiry,Mary I. Carroll,Jessie Hou,Chun Li,Heinz Joseph Lenz,Julie L. Eiseman,James H. Doroshow +12 more
TL;DR: The first-in-human phase I trial of 5-fluoro-2′-deoxycytidine (FdCyd), a DNMT inhibitor stable in aqueous solution, in patients with advanced solid tumors established the safety, maximum tolerated dose (MTD), pharmacokinetics, and pharmacodynamics of F dCyd + tetrahydrouridine (THU).
Journal ArticleDOI
Gene structure and transcription in mouse cells with extensively demethylated DNA.
L A Michalowsky,Peter A. Jones +1 more
TL;DR: There is a subset of methylation sites within these cells which can never be stably demethylated, and this results provide a direct correlation between hypomethylation and the induction of a transcriptionally competent chromatin state.
Journal ArticleDOI
DNA methylation methods: Global DNA methylation and methylomic analyses.
Shizhao Li,Trygve O. Tollefsbol +1 more
TL;DR: The different DNA methylation analysis technologies primarily based on the initial treatments of DNA samples: bisulfite conversion, endonuclease digestion and affinity enrichment, involving methodology evolution, principles, applications, and their relative merits are discussed.
Journal ArticleDOI
Cell cycle effects and cellular pharmacology of 5-aza-2'-deoxycytidine
TL;DR: Results indicate that 5-AZA-CdR produces a preferential kill of cells in the S phase of the cell cycle, which suggests that the cytotoxic action of this analogue is not self-limiting.
References
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A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
Journal ArticleDOI
X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
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Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.