Journal ArticleDOI
Cellular differentiation, cytidine analogs and DNA methylation
Peter A. Jones,Shirley M. Taylor +1 more
Reads0
Chats0
TLDR
Results provide experimental evidence for a role for DNA modification in differentiation, and suggest that cytidine analogs containing an altered 5 position perturb previously established methylation patterns to yield new cellular phenotypes.About:
This article is published in Cell.The article was published on 1980-05-01. It has received 1722 citations till now. The article focuses on the topics: DNA methylation & Zebularine.read more
Citations
More filters
Journal ArticleDOI
Down-regulation of interferon regulatory factor 4 gene expression in leukemic cells due to hypermethylation of CpG motifs in the promoter region
Christina A. Ortmann,Andreas Burchert,Katharina Hölzle,Andreas Nitsche,Burghardt Wittig,Andreas Neubauer,Manuel Schmidt +6 more
TL;DR: CpG site-specific IRF-4 promoter methylation is suggested as a putative mechanism of down-regulated IRf-4 expression in leukemia.
Journal ArticleDOI
A high-throughput screen of inactive X chromosome reactivation identifies the enhancement of DNA demethylation by 5-aza-2′-dC upon inhibition of ribonucleotide reductase
Alissa Minkovsky,Anna Sahakyan,Giancarlo Bonora,Robert Damoiseaux,Elizabeth Dimitrova,Liudmilla Rubbi,Matteo Pellegrini,Caius G. Radu,Kathrin Plath +8 more
TL;DR: This study identifies a drug combination that enhances DNA demethylation by altering nucleotide metabolism by inhibiting ribonucleotide reductase and observing synergistic inhibition of cell growth and a decrease in genome-wide DNA methylation.
Journal Article
DNA Alkali-labile Sites Induced by Incorporation of 5-Aza-2′-deoxycytidine into DNA of Mouse Leukemia L1210 Cells
TL;DR: The effects of 5-aza-2'-deoxycytidine on DNA in mouse L1210 leukemia cells were investigated using the alkaline elution technique and suggests that the alkali lability may be a means by which one could determine the extent of substitution and precise location of azacytosine residues or their ring-opened products in DNA.
Journal ArticleDOI
Tissue-specific DNA methylation patterns of the rat glial fibrillary acidic protein gene
Daniele F. Condorelli,V. G. Nicoletti,Vincenza Barresi,Alessandra Caruso,Silvestro G. Conticello,J. de Vellis,A. M. Giuffrida Stella +6 more
TL;DR: In vivo developmental studies and in vitro differentiation studies are necessary to better understand the functional differences of the various methylatable CpG sites in the 5′ end of the GFAP gene.
Journal ArticleDOI
Effect of combined therapy with low-dose 5-aza-2'-deoxycytidine and irinotecan on colon cancer cell line HCT-15.
Megumi Ishiguro,Satoru Iida,Hiroyuki Uetake,Shinji Morita,Hiroshi Makino,Keiji Kato,Yoko Takagi,Masayuki Enomoto,Kenichi Sugihara +8 more
TL;DR: Pretreatment with low-dose DAC may have the potential to be used as a “biosensitizer” of DNA-damaging agents such as CPT-11 when the apoptotic pathway is inactivated as a result of aberrant promoter methylation in the cancer.
References
More filters
Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
DNA modification mechanisms and gene activity during development.
Robin Holliday,J. E. Pugh +1 more
TL;DR: This article suggests mechanisms that may account for the differentiated state of dividing or nondividing cells and that also attempt to explain the ordered switching on or off of genes during development.
Journal ArticleDOI
X inactivation, differentiation, and DNA methylation.
TL;DR: A model based on DNA methylation is proposed to explain the initiation and maintenance of mammalian X inactivation and certain aspects of other permanent events in eukaryotic cell differentiation using sequence-specific DNA methylases that methylate unmethylated sites with great difficulty but easily methylate half-methylated sites.
Journal ArticleDOI
Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.