Cerebral organoids model human brain development and microcephaly
Madeline A. Lancaster,Magdalena Renner,Carol Anne Martin,Daniel Wenzel,Louise S. Bicknell,Matthew E. Hurles,Tessa Homfray,Josef M. Penninger,Andrew P. Jackson,Juergen A. Knoblich +9 more
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TLDR
A human pluripotent stem cell-derived three-dimensional organoid culture system that develops various discrete, although interdependent, brain regions that include a cerebral cortex containing progenitor populations that organize and produce mature cortical neuron subtypes is developed.Abstract:
The complexity of the human brain has made it difficult to study many brain disorders in model organisms, highlighting the need for an in vitro model of human brain development Here we have developed a human pluripotent stem cell-derived three-dimensional organoid culture system, termed cerebral organoids, that develop various discrete, although interdependent, brain regions These include a cerebral cortex containing progenitor populations that organize and produce mature cortical neuron subtypes Furthermore, cerebral organoids are shown to recapitulate features of human cortical development, namely characteristic progenitor zone organization with abundant outer radial glial stem cells Finally, we use RNA interference and patient-specific induced pluripotent stem cells to model microcephaly, a disorder that has been difficult to recapitulate in mice We demonstrate premature neuronal differentiation in patient organoids, a defect that could help to explain the disease phenotype Together, these data show that three-dimensional organoids can recapitulate development and disease even in this most complex human tissueread more
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Animal and translational models of SARS-CoV-2 infection and COVID-19.
Matt D. Johansen,Aaron T. Irving,X. Montagutelli,Michelle D. Tate,Michelle D. Tate,Ina Rudloff,Ina Rudloff,Marcel F. Nold,Marcel F. Nold,Nicole G. Hansbro,Nicole G. Hansbro,Richard Kim,Richard Kim,Chantal Donovan,Chantal Donovan,Gang Liu,Gang Liu,Alen Faiz,Kirsty R. Short,J. G. Lyons,Geoffrey W. McCaughan,Mark D. Gorrell,Alexander J. Cole,Courtney C. Moreno,David G. Le Couteur,Daniel Hesselson,James A. Triccas,G. Gregory Neely,Jennifer R. Gamble,Stephen J. Simpson,Bernadette M. Saunders,Brian G. Oliver,Brian G. Oliver,Warwick J. Britton,Peter A. B. Wark,Claudia A. Nold-Petry,Claudia A. Nold-Petry,Philip M. Hansbro,Philip M. Hansbro +38 more
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The complexity of tau in Alzheimer's disease.
TL;DR: This review will primarily focus on recent advances in understanding the contributions of tau to AD, as well as new approaches to uncover novel roles of pathological tau during disease progression.
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Biocompatibility of Subcutaneously Implanted Plant-Derived Cellulose Biomaterials.
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Rett syndrome: insights into genetic, molecular and circuit mechanisms.
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Posted ContentDOI
Neuroinvasive potential of SARS-CoV-2 revealed in a human brain organoid model
Eric Song,Ce Zhang,Benjamin Israelow,Alice Lu-Culligan,Alba Sprado,Sophie Skriabine,Peiwen Lu,Orr-El Weizman,Feimei Liu,Yile Dai,Klara Szigeti-Buck,Yuki Yasumoto,Guilin Wang,Christopher Castaldi,Jaime Heltke,Evelyn Ng,John Wheeler,Mia Madel Alfajaro,Etienne Levavasseur,Benjamin Fontes,Neal G. Ravindra,David van Dijk,Shrikant Mane,Murat Gunel,Aaron M. Ring,Syed Kazmi,Kai Zhang,Kai Zhang,Craig B. Wilen,Tamas L. Horvath,Isabelle Plu,Stéphane Haïk,Jean-Leon Thomas,Angeliki Louvi,Shelli F. Farhadian,Anita Huttner,Danielle Seilhean,Nicolas Renier,Kaya Bilguvar,Akiko Iwasaki,Akiko Iwasaki +40 more
TL;DR: It is demonstrated in vivo that SARS-CoV-2 neuroinvasion, but not respiratory infection, is associated with mortality and using mice overexpressing human ACE2, it is demonstrated that neuronal infection can be prevented either by blocking ACE2 with antibodies or by administering cerebrospinal fluid from a COVID-19 patient.
References
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