Journal ArticleDOI
Eine neue Methode zur Synthese von Peptiden: Aktivierung der Carboxylgruppe mit Dicyclohexylcarbodiimid unter Zusatz von 1‐Hydroxy‐benzotriazolen
Wolfgang König,Rolf Geiger +1 more
TLDR
In this article, the authors show that 1-Hydroxy-benzotriazol and 1-hydroxy-acetyl carbodiimid-methode eignen sich als Zusatze bei der Dicyclohexylcarbodiimids-Methode zur Synthese von Peptiden, verhindern die N-Acyl-harnstoffbildung and fuhren in hoher Ausbeute.Abstract:
1-Hydroxy-benzotriazol sowie verschiedene kernsubstituierte 1-Hydroxy-benzotriazole eignen sich als Zusatze bei der Dicyclohexylcarbodiimid-Methode zur Synthese von Peptiden. Ihr Einflus auf die Racemisierung bei Peptidsynthesen wurde unter Anwendung des gaschromatographischen Racemisierungstests von Weygand u. Mitarbb.2) untersucht. Die neuen Zusatze senken die Racemisierung, verhindern die N-Acyl-harnstoffbildung und fuhren in hoher Ausbeute zu sehr reinen Peptiden.read more
Citations
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Journal ArticleDOI
Effect of the microenvironment on the kinetic properties of immobilized enzymes
TL;DR: The results show that the diffusional restrictions on one hand, and the nature of the microenvironment on the other hand, interact in a dynamic way with the enzyme to determine its properties.
Journal ArticleDOI
β‐ENDORPHIN: SYNTHESIS AND RADIORECEPTOR BINDING ACTIVITY OF βh‐ENDORPHIN‐(1–27) AND ITS ANALOGS
TL;DR: The binding potency of I-IV to rat brain membrane preparations was measured by radioreceptor binding assay and the relative potencies were: beta h-endorphin, 100; I, 30; II, 0.04; III, 90; IV, 0,07.
Journal ArticleDOI
Structural requirements essential for elastin coacervation: favorable spatial arrangements of valine ridges on the three-dimensional structure of elastin-derived polypeptide (VPGVG)n
Iori Maeda,Yoshiteru Fukumoto,Takeru Nose,Yasuyuki Shimohigashi,Takashi Nezu,Yoshihiro Terada,Hiroaki Kodama,Kozue Kaibara,Kouji Okamoto +8 more
TL;DR: Results demonstrated that VPGVG is a structural element essential to achieving the β‐spiral structure required for self‐association followed by coacervation, probably due to the ideal spatial arrangement of the hydrophobic Val residues.
Journal ArticleDOI
Rearrangement of the active ester intermediate during HOBt/EDC amide coupling
TL;DR: In this paper, an active ester derivative of benzotriazol-1-yl 1′-(tert-butyloxycarbonylamino)ferrocene-1 carboxylate (2) was synthesized for peptide coupling.
Journal ArticleDOI
Total synthesis of S-carbamoylmethyl bovine apocytochrome c by segment coupling.
TL;DR: Three peptide segments corresponding to the complete sequence of the 104 amino acid protein bovine apocytochrome c were synthesized by the solid-phase method, and the synthetic product was shown to be identical to a sample derived from native bovines cytochromec by paper or gel electrophoresis, HPLC and by chymotryptic or tryptic map.
References
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Journal ArticleDOI
The Use of Esters of N-Hydroxysuccinimide in Peptide Synthesis
Journal ArticleDOI
t-Butyl Esters of Amino Acids and Peptides and their Use in Peptide Synthesis1
Journal ArticleDOI
Zur Synthese des Glucagons, X. Darstellung der Sequenz 22–29
Erich Wünsch,Fritz Drees +1 more
TL;DR: In this paper, the geschutzte Teilsequenz 22-29 des Glucagons, wird auf zwei verschiedenen Wegen synthetisiert.