Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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Effects of p-Nitrotoluene on Cultured Mesencephalic Neural Stem Cells
TL;DR: C cultured neurospheres were used as a very simple primary assay for screening environmental chemicals for disruption of developmental programming and facilitated differentiation with two distinct morphologies outside the sphere, being neural and glial lineages.
Journal ArticleDOI
Comments on “Evaluation of Estrogenic Activities of Aquatic Herbicides and Surfactants Using a Rainbow Trout Vitellogenin Assay”
Journal ArticleDOI
Apoptosis Inducing and Enhancing Activities of Environmental Estrogenic Compounds
TL;DR: The apoptosis-inducing or/and -enhancing effects of the estrogenic compounds observed here appear to be due to their unidentified properties other than estrogenic activity.
Journal ArticleDOI
Ekolojik Risk Değerlendirmede Ekotoksikogenomik Kavramı ve Verdiği Katkılar
TL;DR: Ekotoksikolojide, ekotokosikogenomik yaklasimlar onemli bir alan olmaya baslamistir as discussed by the authors, bu konudaki son gelismeler bu yinimin diger organizmalara da uygulanabilirligini gostermistir.
Dissertation
The impact of environmental endocrine disrupting compounds on ovine fetal adrenal gland development and function
TL;DR: It is hypothesised that in utero exposure to EDCs within sewage sludge dysregulates the developing ovine fetal adrenal gland and may contribute to the increasing incidence of human metabolic disease.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.