Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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Journal ArticleDOI
Estrogenicity of styrene oligomers and assessment of estrogen receptor binding assays.
Katsutoshi Ohno,Yukimasa Azuma,Katsuhiro Date,Shigeru Nakano,Toru Kobayashi,Yasuhiro Nagao,Toshihiro Yamada +6 more
TL;DR: The ER binding assay is the most standardized and simple test system for the detection of specific mechanisms of estrogenic activity and styrene dimers and trimers did not show statistically significant inhibitory action against the binding of [3H]-17β-estradiol (E2) to ER.
Journal ArticleDOI
Orally administered p -nitrotoluene causes hyperactivity, concomitant with gliosis and impairment of tyrosine hydroxylase immunoreactivity in the rat substantia nigra
Masami Ishido,Rumiko Usu +1 more
TL;DR: It is concluded that p-nitrotoluene via oral exposure route caused rat hyperactivity, concomitant with gliosis and impairment of TH immunoreactivity, most likely due to degeneration of dopaminergic neurons.
Journal ArticleDOI
Evaluation of Endocrine Disrupting Activities of Metabolites of Nonylphenol Polyethoxylates by Yeast Two-Hybrid Assay
TL;DR: NPE-cの活性が認められるようになった, £3.5×10-6相当の高い物質を生成する可能性のあることが示唆された.
Journal ArticleDOI
WITHDRAWN: The potential contribution of endocrine disrupting chemicals to acne
TL;DR: This article has been withdrawn at the request of the author(s) and/or editor and the Publisher apologizes for any inconvenience this may cause.
Journal ArticleDOI
RIFM fragrance ingredient safety assessment, p-tolyl alcohol, CAS Registry Number 589-18-4.
A.M. Api,D. Belsito,S. Biserta,D. Botelho,M. Bruze,G.A. Burton,J. Buschmann,M. A. Cancellieri,M.L. Dagli,M. Date,W. Dekant,C. Deodhar,A.D. Fryer,S. Gadhia,L. Jones,K. Joshi,A. Lapczynski,M. Lavelle,D.C. Liebler,M. Na,D. O'Brien,A. Patel,T.M. Penning,G. Ritacco,F. Rodriguez-Ropero,J. Romine,N. Sadekar,D. Salvito,T.W. Schultz,F. Siddiqi,I.G. Sipes,G. Sullivan,Y. Thakkar,Y. Tokura,S. Tsang +34 more
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.