Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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Interaction of hydroxylated polycyclic aromatic hydrocarbons to estrogen receptor
Kazuichi Hayakawa,Yu Onoda,Chihiro Tachikawa,Morio Yoshita,Akira Toriba,Takayuki Kameda,Ning Tang +6 more
TL;DR: In this paper, the binding activities and estrogenic/antiestrogenic activities of polycyclic aromatic hydrocarbons and hydroxylated PAHs (OHPAHs) having 2 to 6 rings were evaluated by competition assay and yeast two-hybrid assay expressing human estrogen receptor (hER), respectively.
Journal ArticleDOI
Weight-of-the-evidence evaluation of 2,4-D potential for interactions with the estrogen, androgen and thyroid pathways and steroidogenesis
Barbara H. Neal,Mary Sue Marty,Katie Coady,Amy Lavin Williams,Jane P. Staveley,James C. Lamb +5 more
TL;DR: 2,4-D is unlikely to pose a threat from endocrine disruption to wildlife or humans under conditions of real-world exposures, and no studies predict significant endocrine-related toxicity or functional decrements in any species at environmentally relevant concentrations, or at doses below the TSRC that are relevant for human hazard and risk assessment.
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Microarray and gene ontology analyses reveal downregulation of DNA repair and apoptotic pathways in diethylstilbestrol-exposed testicular Leydig cells.
Katsuhiko Warita,Katsuhiko Warita,Tomoko Mitsuhashi,Yoshiaki Tabuchi,Ken-ichi Ohta,Shingo Suzuki,Nobuhiko Hoshi,Takanori Miki,Yoshiki Takeuchi +8 more
TL;DR: The findings suggest that molecular events that occur following DES and E2 treatments differ substantially in Leydig cells, and that the effects of synthetic estrogen and natural estrogen differ more substantially than previously suspected.
Journal ArticleDOI
Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors
Haeng-Seog Lee,Shin-ichi Sasagawa,Shigeaki Kato,Ryouichi Fukuda,Hiroyuki Horiuchi,Akinori Ohta +5 more
TL;DR: A combination of the full-length human estrogen receptor α with the nuclear receptor-binding domain of co-Activator steroid receptor co-activator-1 or transcriptional intermediate factor-2 (TIF-2) was most effective for estrogen-dependent induction of the chromosome-integrated UASGAL-CYC1 p-lacZ reporter construct among the two-hybrid systems so far tested.
Journal ArticleDOI
Pronamide: Weight of evidence for potential estrogen, androgen or thyroid effects.
Mary Sue Marty,Sabitha Papineni,Katherine K. Coady,Reza J. Rasoulpour,Lynn H. Pottenger,David L. Eisenbrandt +5 more
TL;DR: While pronamide alters some endocrine-sensitive endpoints in EDSP Tier 1 assays, effects on liver metabolism likely explain altered hormone levels and indirect endocrine changes.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.