Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
Reads0
Chats0
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
More filters
Journal ArticleDOI
Occurrence and Human Exposure of p-Hydroxybenzoic Acid Esters (Parabens), Bisphenol A Diglycidyl Ether (BADGE), and Their Hydrolysis Products in Indoor Dust from the United States and Three East Asian Countries
Lei Wang,Chunyang Liao,Fang Liu,Qian Wu,Ying Guo,Hyo-Bang Moon,Haruhiko Nakata,Kurunthachalam Kannan +7 more
TL;DR: This is the first report of BADGE and its hydrolysis products (BADGEs) in indoor dust samples and of parabens in indoorDust from Asian countries and the daily intake (EDI) via dust ingestion were 5-10 times higher in children than in adults.
Journal ArticleDOI
Oestrogenic activity of benzylparaben.
Philippa D. Darbre,J. R. Byford,L. E. Shaw,S. Hall,Nick G. Coldham,G. S. Pope,Maurice J. Sauer +6 more
TL;DR: It is demonstrated that the oestrogenicity of methylparaben can be increased by the addition of an aryl group as well as by lengthening or branching the alkyl grouping.
Journal ArticleDOI
In vitro profiling of the endocrine disrupting potency of organochlorine pesticides.
TL;DR: The results suggested that, for the tested OCPs, their ability to act as endocrine disruptors involves more than one mechanism, their (anti-)agonistic effects on different receptors should not be overlooked, and the potential for additive or synergistic effects must be taken into consideration in the risk assessment process.
Journal ArticleDOI
Oestrogenic activity of p-hydroxybenzoic acid (common metabolite of paraben esters) and methylparaben in human breast cancer cell lines.
TL;DR: It can be concluded that removal of the ester group from parabens does not abrogate its oestrogenic activity and that p‐hydroxybenzoic acid can give oestrogensic responses in human breast cancer cells.
Journal ArticleDOI
Using in Vitro High Throughput Screening Assays to Identify Potential Endocrine-Disrupting Chemicals
Daniel M. Rotroff,David J. Dix,Keith A. Houck,Thomas B. Knudsen,Matthew T. Martin,Keith W. McLaurin,David M. Reif,Kevin M. Crofton,Amar V. Singh,Menghang Xia,Ruili Huang,Richard S. Judson +11 more
TL;DR: Overall, results suggest that current ToxCast assays can accurately identify chemicals with potential to interact with the estrogenic and androgenic pathways, and could help prioritize chemicals for EDSP T1S assays.
References
More filters
Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.