Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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Chlorination of bisphenol S: Kinetics, products, and effect of humic acid.
Yuan Gao,Jin Jiang,Yang Zhou,Su-Yan Pang,Jun Ma,Chengchun Jiang,Yang Yue,Zhuangsong Huang,Jia Gu,Qin Guo,Jiebin Duan,Juan Li +11 more
TL;DR: It was found that free chlorine could effectively degrade bisphenol S over a wide pH range from 5 to 10 with apparent second-order rate constants of 7.6-435.3 M-1s-1.
Journal ArticleDOI
Morphometric analysis of mice uteri treated with the preservatives methyl, ethyl, propyl, and butylparaben
TL;DR: Pbens at the doses assayed here induce estrogenic histological changes in the uteri of Ovx mice, and evidence of their estrogenicity is presented for the first time.
Journal ArticleDOI
In silico approach to evaluate molecular interaction between mycotoxins and the estrogen receptors ligand binding domain: a case study on zearalenone and its metabolites.
Pietro Cozzini,Luca Dellafiora +1 more
TL;DR: It is demonstrated that the study on the interactions of unsuspected molecules with estrogen receptors, using the same technique applied in medicinal chemistry could be a valuable choice to discover new hypothetical xenoestrogens.
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Global review of phthalates in edible oil: An emerging and nonnegligible exposure source to human
TL;DR: This work provided the first evidence that edible oil is a potential source of phthalates, thus the potential adverse estrogenic effects on human health should need to be assessed in a holistic manner.
Journal ArticleDOI
Liquid chromatography-bioassay-mass spectrometry for profiling of physiologically active food.
TL;DR: A novel effect-directed food profiling is shown, as food and food supplements can be unknowingly physiologically active and endocrine compounds in food were quantitatively detected as sharp-bounded zones and further characterized by mass spectrometry.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.