Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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Journal ArticleDOI
Evaluation of estrogenic activities of hydroxylated polycyclic aromatic hydrocarbons in cigarette smoke condensate.
TL;DR: This is a first study to identify estrogenic hydroxylated PAHs in cigarette smoke condensate and points out the necessity for detailed investigation of exposure to aerosols containing apparently estrogenic compounds.
Journal ArticleDOI
In vitro and in vivo estrogenicity and toxicity of o-, m-, and p-dichlorobenzene
TL;DR: Elevated VTG levels and decreased female GSIs were found in fish exposed to ≥5 ng EE2/L and in fish exposure to ≥10 mg p‐DCB/L, and a direct or indirect effect of VTG on the GSI is suggested rather than a direct toxic effect of the tested compounds on the gonads.
Journal ArticleDOI
An in silico ensemble method for lead discovery: decision forest.
TL;DR: A decision forest model was developed using a structurally diverse training data set containing 232 compounds whose estrogen receptor binding activity was tested at the U.S. Food and Drug Administration (FDA)'s National Center for Toxicological Research (NCTR).
Journal ArticleDOI
Effects of a diphenyl ether-type herbicide, chlornitrofen, and its amino derivative on androgen and estrogen receptor activities.
TL;DR: The results suggest that both CNP and CNP-amino may act as endocrine disruptors via AR and ER alpha in humans and other animals.
Journal ArticleDOI
Lack of (anti-) androgenic or estrogenic effects of three pyrethroids (esfenvalerate, fenvalerate, and permethrin) in the Hershberger and uterotrophic assays.
Takeshi Kunimatsu,Tomoya Yamada,Keiko Ose,Osamu Sunami,Yusuke Kamita,Yasuyoshi Okuno,Takaki Seki,Iwao Nakatsuka +7 more
TL;DR: Evaluated the interaction of three pyrethroids with androgen receptor (AR)- and estrogen receptor (ER)-mediated mechanisms using in vivo short-term assays and concluded that none of esfenvalerate, fenavalerate, or permethrin exhibit any potential to cause adverse (anti-) androgenic or estrogenic effects at dose levels below that of those causing excessive systemic toxicity.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.