Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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Effect of UV screens and preservatives on vitellogenin and choriogenin production in male medaka (Oryzias latipes)
Madoka Inui,Tetsuya Adachi,Shigeo Takenaka,Hiroshi Inui,Masami Nakazawa,Mitsuhiro Ueda,Hajime Watanabe,Chisato Mori,Taisen Iguchi,Kazutaka Miyatake +9 more
TL;DR: It is shown that 4-MBC, OMC, and PP have estrogenic activity in fish, and increased mRNA expression levels of estrogen receptor (ER) alpha, among sex hormone receptors in the liver, are found due to exposure to 5-methyl-benzylidene camphor, among UV screens and preservatives.
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Emerging contaminants in surface waters and their relevance for the production of drinking water in Europe
TL;DR: An overview of emerging contaminants in the aquatic environment that are of relevance for drinking water production can be found in this paper, where the individual compounds are observed in concentrations that are generally considered too low to cause acute effects.
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Toxication or detoxication? In vivo toxicity assessment of ozonation as advanced wastewater treatment with the rainbow trout.
TL;DR: The results indicate that in any case ozonation should not be applied without subsequent post treatment appropriate for oxidation byproducts removal (e.g. sand filtration).
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Endocrine disrupting effects of herbicides and pentachlorophenol: in vitro and in vivo evidence.
TL;DR: Novel effects of herbicides and PCP at environmentally relevant concentrations were found, and the effects of these compounds on humans and/or wildlife warrant further investigation.
Journal ArticleDOI
Widely used pesticides with previously unknown endocrine activity revealed as in vitro antiandrogens.
TL;DR: Due to estimated antiandrogenic potency, current use, estimated exposure, and lack of previous data, it is strongly recommend that dimethomorph, fludioxonil, fenhexamid, imazalil, ortho-phenylphenol, and pirimiphos-methyl be tested for anti androgenic effects in vivo.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.