Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
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Introduction and summary of findings: Chapter 1 in A synthesis of aquatic science for management of Lakes Mead and Mohave
Journal ArticleDOI
Estrogenic Activity of Alkyl(thio)phenols and 4,4'-thiodiphenol Formed from Degradation of Commercial Insecticides
TL;DR: In this paper, the estrogenic activity of commercial insecticides fenamiphos, fenthion, methiocarb, propaphos, sulprophos, and temephos as well as some phenolic compounds obtained as a result of their degradation was investigated.
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The Potential Contribution of Phytoestrogens and Organochlorine Pesticides in an Experimental Fish Diet to Estrogenic Activity
Munekazu Matsuoka,Hiroshi Ishibashi,Marie Ushijima,Makiko Inudo,Eiko Honda,Masayoshi Iwahara,Hyeon Seo Cho,Yasuhiro Ishibashi,Koji Arizono +8 more
TL;DR: Results indicate that phytoestrogens, such as genistein and daidzein, were the main substances contributing to the estrogenic activity of the diet, indicating the necessity for more careful selection of the feeding diet and measurement of estrogenic substances when performing routine screening assays for endocrine-disrupting chemicals.
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Modified Yeast Two-hybrid Assay to Evaluate the Estrogenic Activity of Environmental Samples.
TL;DR: A yeast two-hybrid system appears more simple and inexpensive assay with high repeatability, however, the biggest problem of this bioassay is that it cannot measure estrogenic activity in some of the environmental samples.
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Development of Plasma Vitellogenin Assay for Estrogenic Effects of Endocrine-Disrupting Chemicals Using Ovariectomized Goldfish (Carassius auratus)
Hiroshi Ishibashi,Hiroshi Ishibashi,Makito Kobayashi,Yuki Tomiyasu,Maki Miyahara,Katsuyasu Tachibana,Mutsuyosi Tsuchimoto,Koji Arizono +7 more
TL;DR: The results indicate that the estrogen activity of CD may be high enough to cause induction of plasma VTG in ovariectomized goldfish as previously observed in male goldfish, and suggests that induction of Plasma VTG using ovariectomy goldfish is a good tool for evaluating the estrogenic effects of endocrine-disrupting chemicals.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.