Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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Journal ArticleDOI
Estrogenic effects of marijuana smoke condensate and cannabinoid compounds.
TL;DR: The results suggest that marijuana abuse is considered an endocrine-disrupting factor, and suggest that the phenolic compounds contained in MSC play a role in its estrogenic effect.
Journal ArticleDOI
Formation of estrogenic products from environmental phthalate esters under light exposure.
Yoshinori Okamoto,Tomohiro Hayashi,Chitose Toda,Koji Ueda,Kiyomatsu Hashizume,Kazuo Itoh,Jun-ichi Nishikawa,Tsutomu Nishihara,Nakao Kojima +8 more
TL;DR: The proposed mechanism for PE-4OH production is such that by PE-mediated photosensitization H2O2 is generated from O2 and H+ and decomposed to hydroxyl radical, thus oxidizing the PE benzene ring.
Journal ArticleDOI
Analysis of protein tyrosine kinase inhibitors in recombinant yeast lacking the ERG6 gene.
TL;DR: The construction of the novel Saccharomyces cerevisiae yeast strain DCY250 is reported, which is compatible with yeast two‐hybrid‐based systems and bears a targeted disruption of the ERG6 gene to ablate ergosterol biosynthesis and enhance permeability to small molecules.
Journal ArticleDOI
NTP Research Report on Biological Activity of Bisphenol A (BPA) Structural Analogues and Functional Alternatives
Katherine E. Pelch,Jessica A. Wignall,Alexandra E. Goldstone,Pam K. Ross,Robyn B. Blain,Andrew J. Shapiro,Stephanie Holmgren,Jui-Hua Hsieh,Daniel L. Svoboda,Scott S. Auerbach,Frederick M. Parham,Scott A. Masten,Kristina A. Thayer +12 more
TL;DR: Analysis of data available from the high throughput screening programs Tox21/ToxCast showed that in general, BPA analogues and derivatives are more structurally and biologically similar to BPA, and to each other, than to E2.
Journal ArticleDOI
Study on interactions of endocrine disruptors with estrogen receptor using fluorescence polarization.
Ken-ichi Ohno,Ken-ichi Ohno,Sachiko Suzuki,Takeshi Fukushima,Masako Maeda,Tomofumi Santa,Kazuhiro Imai +6 more
TL;DR: The tested compounds were classified into three categories, agonists, partial agonists and antagonists based on their Hill coefficients, and the relationship between the chemical structures and estrogenic activities was examined.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.