Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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Journal ArticleDOI
Oligomers of styrene are not endocrine disruptors
Heinz-Peter Gelbke,Marcy I. Banton,Christian Block,Gordon Dawkins,Edgar Leibold,Mark Pemberton,Atsunobu Sakoda,Atsushi Yasukawa +7 more
TL;DR: The integration of the total information supports the conclusion that neither specific oligomers, nor their mixtures, potentially migrating into food are endocrine disruptors according to the definition of EFSA and WHO/IPCS.
Journal ArticleDOI
Dimerization of Bisphenol A by Hyper Lignin-Degrading Fungus Phanerochaete sordida YK-624 Under Ligninolytic Condition
TL;DR: Results indicated that BPA was oxidized to BPA phenoxy radicals by ligninolytic enzymes and then dimerized at extracellular region.
Journal ArticleDOI
Identification of oxidative stress and estrogenic activity induced by polarity fractions of effluent organic matter.
Jisu Yoo,Jin Hur,Jinho Jung +2 more
TL;DR: Findings suggest that aromatic protein-like substances and SMPs in EfOM were responsible for the observed oxidative stress and estrogenic activity.
Dissertation
Unconventional oil and gas extraction as a novel source of endocrine disrupting chemicals to water and the potential for adverse human and animal health outcomes
TL;DR: In this paper, the authors propose a method to solve the problem of homonymity in homonym identification, i.e., homonymization, in the context of homology.
Journal ArticleDOI
Dose-dependent acceleration in the delayed effects of neonatal oral exposure to low-dose 17α-ethynylestradiol on reproductive functions in female Sprague-Dawley rats
TL;DR: It is concluded that a slight increase in the circulating estrogens during the neonatal period exerts irreversible delayed effects.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.