Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
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Marijuana extracts possess the effects like the endocrine disrupting chemicals.
Kazuhito Watanabe,Erina Motoya,Naoki Matsuzawa,Tatsuya Funahashi,Toshiyuki Kimura,Tamihide Matsunaga,Koji Arizono,Ikuo Yamamoto +7 more
TL;DR: The results indicate that there are some metabolic interactions between cannabinoid and steroid metabolism and that the constituents showing estrogen-like activity exist in marijuana.
Journal ArticleDOI
Estrogenic and antiestrogenic activities of the roots of Moghania philippinensis and their constituents.
TL;DR: Through estrogenic activity-guided fractionation, several active flavonoids including prenylated ones were isolated and 5,7,3',4'-Tetrahydroxy-6,8-diprenylisoflavone showed the strongest antiestrogenic activity.
BookDOI
In Silico Methods for Predicting Drug Toxicity
TL;DR: The basis of computational chemistry is introduced, how computational methods have been extended to some biological properties and toxicology, and how animal experiments can be mimicked by new in silico methods.
Journal ArticleDOI
Effects of in utero exposure to di-n-hexyl phthalate on the reproductive development of the male rat.
TL;DR: Results showed that prenatal exposure to DnHP caused permanent and dose-related alterations of the male rat reproductive development, with a similar profile as DEHP.
Journal ArticleDOI
Estrogens in the daily diet: In vitro analysis indicates that estrogenic activity is omnipresent in foodstuff and infant formula
TL;DR: The results demonstrate that many food types are a source of unidentified estrogen-like compounds still awaiting toxicological evaluation, and conclude that dietary estrogens are omnipresent and not limited to soy-based food.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.