Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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Journal Article
Organochlorines and endometriosis: a mini literature review
TL;DR: There is accumulative evidence from the individual studies that high concentrations of organochlorine compounds are not important predictors of endometriosis and the role of health care professionals should be focused on the education of the public in order to minimize their exposure toorganochlorines and other harmful substances.
Journal ArticleDOI
Effect of Estrogenic Compounds on Superoxide and Nitric Oxide Production by Activated Macrophages Assessed by Sensitive Microplate Assays
Wataru Yoshida,Minori Ogawa,Yuko Furuoya,Yu Igarashi,Rumi Sato,Akiko Hayashi,Kazuya Hirano,Masatoshi Beppu +7 more
TL;DR: It is suggested that bisphenol A and genistein similarly affect superoxide and nitric oxide production by a mechanism other than that involving estrogenic activity.
Proceedings ArticleDOI
Development of single cell biochip platform based on silicon for multiple patch clamping technology
TL;DR: The cell attachment technique to grab the cell successfully and the N/MEMS process to make a micrometer hole in one of an array of electrode for the cell clamp are presented.
Journal ArticleDOI
Structure-activity relationships and pathway analysis of biological degradation processes
TL;DR: Standard (Q)SAR methods and biological database construction are discussed as well as an example of how SAR and metabolic pathway analysis can be combined to examine the biological degradation processes of endocrine disrupting chemicals.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.