Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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Metabolic Activation of Proestrogens in the Environment by Cytochrome P450 System
TL;DR: Liver microsome-mediated activation of proestrogens in the environment is reviewed here and various candidates for proestrogenic compounds among medicines and insecticides are discussed.
Journal ArticleDOI
Multiplex planar bioassay detecting estrogens, antiestrogens, false-positives and synergists as sharp zones on normal phase.
TL;DR: In this article , a planar yeast estrogen screen (pYES) is extended to a multiplex bioassay, offering 4 different biological activity principles, followed by targeted identification of active zones.
Journal ArticleDOI
Development of a Competitive Enzyme Immunoassay for Detection of Capacity of Chemicals to Bind Quail Estrogen Receptor α and β
Shinobu Maekawa,Makoto Nishizuka,Shiro Heitaku,Masaaki Kunimoto,Jun-ichi Nishikawa,Kouhei Ichikawa,Kiyoshi Shimada,Masayoshi Imagawa +7 more
TL;DR: The ligand binding domain (LBD) of quail ERα and ERβ is expressed as a fusion protein with glutathione S-transferase and using these proteins, two systems for assaying the capacity to bind ERs in vitro are developed.
Journal ArticleDOI
Mutagenicities and Endocrine-disrupting Activities of 1-Hydroxy-2-nitropyrene and 1-Hydroxy-5-nitropyrene
Takayuki Kameda,Ayuko Akiyama,Morio Yoshita,Chihiro Tachikawa,Akira Toriba,Ning Tang,Kazuichi Hayakawa +6 more
TL;DR: The mutagenicities and endocrine-disrupting activities of two isomers of mononitrated 1-hydroxypyrene, which are not only photoreaction products of 1-nitropyrene but also constituent of ambient airborne particles, were evaluated for the first time using the Ames plate incorporation assay and the yeast two-hybrid assay.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.