Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
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The identification of readily bioavailable pollutants in Lake Shkodra/Skadar using semipermeable membrane devices (SPMDs), bioassays and chemical analysis.
Andrew Rastall,Andrew Rastall,Anila Neziri,Zeljko Vukovic,Christine Jung,S. Mijovic,Henner Hollert,Svetlana Nikcevic,Lothar Erdinger +8 more
TL;DR: The combination of SPMD-based sampling with appropriate bioassays and chemical analysis provided an effective tool for the identification of environmentally relevant waterborne pollutants in Lake Shkodra/Skadar and showed that toxicologically relevant HOPs including EROD-inducing and potentially estrogenic compounds are widely distributed in the lake and readily available for uptake by aquatic biota.
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Anti-estrogenic activity of fifty chemicals evaluated by in vitro assays.
TL;DR: It is suggested that three of fifty chemicals could inhibit estrogen activity by competitive binding with 17beta-estradiol to the estrogen receptor.
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Association between paraben exposure and menstrual cycle in female university students in Japan.
Yukiko Nishihama,Jun Yoshinaga,Jun Yoshinaga,Ayaka Iida,Shoko Konishi,Shoko Konishi,Hideki Imai,Miyuki Yoneyama,Daisuke Nakajima,Hiroaki Shiraishi +9 more
TL;DR: It is indicated that paraben exposure at not excessive levels is associated with menstrual cycle length or its variability among general female subjects, suggesting a possibility thatParabens exposure is one of the environmental causes of human reproductive problem.
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Evaluating the interactions of vertebrate receptors with persistent pollutants and antifouling pesticides using recombinant yeast assays
Tania-Noelia Noguerol,Susanna Boronat,Marta Casado,Demetrio Raldúa,Damià Barceló,Benjamin Piña +5 more
TL;DR: This work uses two yeast systems to test the ability of different chemicals to bind and activate two vertebrate receptors which are intimately related to adverse biological effects of pollution in exposed fauna: the estrogen receptor (ER) and the aryl hydrocarbon receptor (AhR).
Journal ArticleDOI
Biodegradation of bisphenol A by bacterial consortia
TL;DR: It is demonstrated that bacterial consortia may assemble a wide range of catabolic pathways to allow for efficient degradation of BPA, converting BPA to principally bacterial biomass and metabolites exhibiting low or no oestrogenic activity.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.