Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
Reads0
Chats0
TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
Citations
More filters
OtherDOI
Effects of wastewater discharges on endocrine and reproductive function of Western Mosquitofish (Gambusia spp.) and implications for the threatened Santa Ana Sucker (Catostomus santaanae)
Jill A. Jenkins,Steven L. Goodbred,Heather M. Olivier,Rassa O. Draugelis-Dale,David A. Alvarez +4 more
TL;DR: This document summarizes current capabilities, research and operational priorities, and plans for further studies that were established at the 2015 USGS workshop on quantitative hazard assessments of earthquake-triggered landsliding and liquefaction in the Czech Republic.
Journal ArticleDOI
Estrogenic effects of dissolved organic matter and its impact on the activity of 17β-estradiol
TL;DR: Though direct estrogenic effects of DOM were not observed, increase in the estrogenic activity of DOM after irradiation was significant, and DOM shows amphoteric influence on the natural estrogen E2, which depends on the concentration of DOM used.
OtherDOI
Investigation of Organic Chemicals Potentially Responsible for Mortality and Intersex in Fish of the North Fork of the Shenandoah River, Virginia, during Spring of 2007
David A. Alvarez,Walter L. Cranor,Stephanie D. Perkins,Vickie L. Schroeder,Stephen L. Werner,Edward T. Furlong,John Holmes +6 more
TL;DR: In this article, the authors propose a method to solve the problem of homonymity in homonym identification, i.e., homonym-of-individuals-with-groups.
Journal ArticleDOI
QSAR-assisted design of an environmental catalyst for enhanced estrogen remediation.
TL;DR: The novel QSAR-assisted approach described herein offers specific advantages compared to conventional design strategies, most notably targeting an entire class of pollutants at one time and a flexible hybridization of benefits associated with rational design and directed evolution.
References
More filters
Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.