Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells
Steven A. Sloan,Spyros Darmanis,Nina Huber,Themasap Khan,Fikri Birey,Christine Caneda,Richard J. Reimer,Stephen R. Quake,Ben A. Barres,Sergiu P. Paşca +9 more
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TLDR
It is found that hCS-derived glia closely resemble primary human fetal astrocytes and that, over time in vitro, they transition from a predominantly fetal to an increasingly matureAstrocyte state.About:
This article is published in Neuron.The article was published on 2017-08-16 and is currently open access. It has received 385 citations till now. The article focuses on the topics: Astrocyte & Induced pluripotent stem cell.read more
Citations
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Reactive astrocyte nomenclature, definitions, and future directions
Carole Escartin,Elena Galea,Andras Lakatos,James P. O'Callaghan,Gabor C. Petzold,Gabor C. Petzold,Alberto Serrano-Pozo,Christian Steinhäuser,Andrea Volterra,Giorgio Carmignoto,Giorgio Carmignoto,Amit Agarwal,Nicola J. Allen,Alfonso Araque,Luis Barbeito,Ari Barzilai,Dwight E. Bergles,Gilles Bonvento,Arthur M. Butt,Wei Ting Chen,Martine Cohen-Salmon,Colm Cunningham,Benjamin Deneen,Bart De Strooper,Bart De Strooper,Blanca Diaz-Castro,Cinthia Farina,Marc R. Freeman,Vittorio Gallo,James E. Goldman,Steven A. Goldman,Steven A. Goldman,Magdalena Götz,Antonia Gutierrez,Philip G. Haydon,Dieter Henrik Heiland,Elly M. Hol,Matthew Holt,Masamitsu Iino,Ksenia V. Kastanenka,Helmut Kettenmann,Baljit S. Khakh,Schuichi Koizumi,C. Justin Lee,Shane A. Liddelow,Brian A. MacVicar,Pierre J. Magistretti,Pierre J. Magistretti,Albee Messing,Anusha Mishra,Anna V. Molofsky,Keith K. Murai,Christopher M. Norris,Seiji Okada,Stéphane H. R. Oliet,João Filipe Oliveira,João Filipe Oliveira,Aude Panatier,Vladimir Parpura,Marcela Pekna,Milos Pekny,Luc Pellerin,Gertrudis Perea,Beatriz G. Pérez-Nievas,Frank W. Pfrieger,Kira E. Poskanzer,Francisco J. Quintana,Richard M. Ransohoff,Miriam Riquelme-Perez,Stefanie Robel,Christine R. Rose,Jeffrey D. Rothstein,Nathalie Rouach,David H. Rowitch,Alexey Semyanov,Alexey Semyanov,Swetlana Sirko,Harald Sontheimer,Raymond A. Swanson,Javier Vitorica,Ina B. Wanner,Levi B. Wood,Jia Qian Wu,Binhai Zheng,Eduardo R. Zimmer,Robert Zorec,Michael V. Sofroniew,Alexei Verkhratsky,Alexei Verkhratsky +88 more
TL;DR: In this article, the authors point out the shortcomings of binary divisions of reactive astrocytes into good-vs-bad, neurotoxic vs-neuroprotective or A1-vs.A2.
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Cell Biology of Astrocyte-Synapse Interactions
Nicola J. Allen,Cagla Eroglu +1 more
TL;DR: This review summarizes some of the seminal findings that yield important insight into the cellular and molecular basis of astrocyte-neuron communication and poses some pressing questions that need to be addressed to advance mechanistic understanding of the role ofAstrocytes in regulating synaptic development.
Journal ArticleDOI
Organoid single-cell genomic atlas uncovers human-specific features of brain development
Sabina Kanton,Michael Boyle,Zhisong He,Zhisong He,Malgorzata Santel,Anne Weigert,Fátima Sanchís-Calleja,Fátima Sanchís-Calleja,Patricia Guijarro,Leila Sidow,Jonas Simon Fleck,Dingding Han,Zhengzong Qian,Michael Heide,Wieland B. Huttner,Philipp Khaitovich,Philipp Khaitovich,Philipp Khaitovich,Svante Pääbo,Barbara Treutlein,Barbara Treutlein,J. Gray Camp +21 more
TL;DR: The data provide a temporal cell atlas of great ape forebrain development, and illuminate dynamic gene-regulatory features that are unique to humans, using single-cell transcriptomics and accessible chromatin profiling in stem cell-derived cerebral organoids to investigate gene-Regulatory changes that are specific to humans.
Journal ArticleDOI
Establishing Cerebral Organoids as Models of Human-Specific Brain Evolution.
Alex A. Pollen,Aparna Bhaduri,Madeline G. Andrews,Tomasz J. Nowakowski,Olivia S. Meyerson,Mohammed A. Mostajo-Radji,Elizabeth Di Lullo,Beatriz Alvarado,Melanie Bedolli,Max L. Dougherty,Ian T. Fiddes,Zev N. Kronenberg,Joe Shuga,Anne A. Leyrat,Jay A. A. West,Marina Bershteyn,Craig B. Lowe,Bryan J Pavlovic,Sofie R. Salama,David Haussler,David Haussler,Evan E. Eichler,Arnold R. Kriegstein +22 more
TL;DR: This work leveraged recent innovations that permit generating pluripotent stem cell-derived cerebral organoids from chimpanzee to identify human-specific features of cortical development and identifies 261 differentially expressed genes in human compared to both chimpanzee organoids and macaque cortex.
Journal ArticleDOI
Induced pluripotent stem cells in disease modelling and drug discovery.
R. Grant Rowe,George Q. Daley +1 more
TL;DR: This Review summarizes the progress in iPSC-based disease modelling over the past decade, with a focus on 3D organoid systems and chimeric models being exploited for new therapeutic approaches.
References
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Journal ArticleDOI
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
Journal Article
Visualizing Data using t-SNE
TL;DR: A new technique called t-SNE that visualizes high-dimensional data by giving each datapoint a location in a two or three-dimensional map, a variation of Stochastic Neighbor Embedding that is much easier to optimize, and produces significantly better visualizations by reducing the tendency to crowd points together in the center of the map.
Journal ArticleDOI
Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation
Cole Trapnell,Cole Trapnell,Brian A. Williams,Geo Pertea,Ali Mortazavi,Gordon Kwan,Marijke J. van Baren,Steven L. Salzberg,Barbara J. Wold,Lior Pachter +9 more
TL;DR: The results suggest that Cufflinks can illuminate the substantial regulatory flexibility and complexity in even this well-studied model of muscle development and that it can improve transcriptome-based genome annotation.
Journal ArticleDOI
TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions
Daehwan Kim,Daehwan Kim,Geo Pertea,Cole Trapnell,Cole Trapnell,Harold Pimentel,Kelley Ryan Matthew,Steven L. Salzberg,Steven L. Salzberg +8 more
TL;DR: TopHat2 is described, which incorporates many significant enhancements to TopHat, and combines the ability to identify novel splice sites with direct mapping to known transcripts, producing sensitive and accurate alignments, even for highly repetitive genomes or in the presence of pseudogenes.
Journal ArticleDOI
The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells
Cole Trapnell,Davide Cacchiarelli,Davide Cacchiarelli,Jonna Grimsby,Prapti Pokharel,Shuqiang Li,Michael A. Morse,Michael A. Morse,Niall J. Lennon,Kenneth J. Livak,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,John L. Rinn,John L. Rinn,John L. Rinn +14 more
TL;DR: Monocle is described, an unsupervised algorithm that increases the temporal resolution of transcriptome dynamics using single-cell RNA-Seq data collected at multiple time points that revealed switch-like changes in expression of key regulatory factors, sequential waves of gene regulation, and expression of regulators that were not known to act in differentiation.
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