Longitudinal Isolation of Potent Near-Germline SARS-CoV-2-Neutralizing Antibodies from COVID-19 Patients.
Christoph Kreer,Matthias Zehner,Timm Weber,Meryem S. Ercanoglu,Lutz Gieselmann,Cornelius Rohde,Sandro Halwe,M. Korenkov,Philipp Schommers,Kanika Vanshylla,Veronica Di Cristanziano,Hanna Janicki,Reinhild Brinker,Artem Ashurov,Verena Krähling,Alexandra Kupke,Hadas Cohen-Dvashi,Manuel Koch,Jan Mathis Eckert,Simone Lederer,Nico Pfeifer,Timo Wolf,Maria J G T Vehreschild,Clemens M. Wendtner,Ron Diskin,Henning Gruell,Stephan Becker,Florian Klein +27 more
Reads0
Chats0
TLDR
It is demonstrated that SARS-CoV-2-neutralizing antibodies are readily generated from a diverse pool of precursors, fostering hope for rapid induction of a protective immune response upon vaccination.About:
This article is published in Cell.The article was published on 2020-08-20 and is currently open access. It has received 329 citations till now. The article focuses on the topics: Neutralizing antibody & Monoclonal antibody.read more
Citations
More filters
Journal ArticleDOI
SARS-CoV-2 neutralizing antibody structures inform therapeutic strategies.
Christopher O. Barnes,Claudia A. Jette,Morgan E. Abernathy,Kim Marie A. Dam,Shannon R. Esswein,Harry B. Gristick,Andrey G. Malyutin,Naima G. Sharaf,Kathryn E. Huey-Tubman,Yu E. Lee,Davide F. Robbiani,Davide F. Robbiani,Michel C. Nussenzweig,Michel C. Nussenzweig,Anthony P. West,Pamela J. Bjorkman +15 more
TL;DR: Eight new structures of distinct COVID-19 human neutralizing antibodies 5 in complex with the SARS-CoV-2 spike trimer or RBD are solved and rules for assigning current and future human RBD-targeting antibodies into classes, evaluating avidity effects and suggesting combinations for clinical use are provided.
Journal ArticleDOI
Escape from neutralizing antibodies by SARS-CoV-2 spike protein variants.
Yiska Weisblum,Fabian Schmidt,Fengwen Zhang,Justin DaSilva,Daniel Poston,Julio C. C. Lorenzi,Frauke Muecksch,Magdalena Rutkowska,Hans-Heinrich Hoffmann,Eleftherios Michailidis,Christian Gaebler,Marianna Agudelo,Alice Cho,Zijun Wang,Anna Gazumyan,Melissa Cipolla,Larry L. Luchsinger,Christopher D. Hillyer,Marina Caskey,Davide F. Robbiani,Davide F. Robbiani,Charles M. Rice,Michel C. Nussenzweig,Michel C. Nussenzweig,Theodora Hatziioannou,Paul D. Bieniasz,Paul D. Bieniasz +26 more
TL;DR: It is shown that functional SARS-CoV-2 S protein variants with mutations in the receptor-binding domain (RBD) and N-terminal domain that confer resistance to monoclonal antibodies or convalescent plasma can be readily selected.
Journal ArticleDOI
Evolution of antibody immunity to SARS-CoV-2.
Christian Gaebler,Zijun Wang,Julio C. C. Lorenzi,Frauke Muecksch,Shlomo Finkin,Minami Tokuyama,Alice Cho,Mila Jankovic,Dennis Schaefer-Babajew,Thiago Y. Oliveira,Melissa Cipolla,Charlotte Viant,Christopher O. Barnes,Yaron Bram,Gaëlle Breton,Thomas Hagglof,Pilar Mendoza,Arlene Hurley,Martina Turroja,Kristie Gordon,Katrina G. Millard,Victor A. Ramos,Fabian Schmidt,Yiska Weisblum,Divya Jha,Michael Tankelevich,Gustavo Martinez-Delgado,Jim Yee,Roshni Patel,Juan Dizon,Cecille Unson-O'Brien,Irina Shimeliovich,Davide F. Robbiani,Zhen Zhao,Anna Gazumyan,Robert E. Schwartz,Theodora Hatziioannou,Pamela J. Bjorkman,Saurabh Mehandru,Paul D. Bieniasz,Paul D. Bieniasz,Marina Caskey,Michel C. Nussenzweig,Michel C. Nussenzweig +43 more
TL;DR: In this article, the authors report on the humoral memory response in a cohort of 87 individuals assessed at 1.3 and 6.2 months after infection with SARS-CoV-2.
Journal ArticleDOI
Persistence of serum and saliva antibody responses to SARS-CoV-2 spike antigens in COVID-19 patients.
Baweleta Isho,Kento T. Abe,Kento T. Abe,Michelle Zuo,Alainna J Jamal,Alainna J Jamal,Bhavisha Rathod,Jenny Wang,Zhijie Li,Gary Chao,Olga L. Rojas,Yeo Myong Bang,Annie Pu,Natasha Christie-Holmes,Christian Gervais,Derek F. Ceccarelli,Payman Samavarchi-Tehrani,Furkan Guvenc,Patrick Budylowski,Angel Li,Aimee Paterson,Feng Yun Yue,Lina María Marín,Lauren Caldwell,Jeffrey L. Wrana,Jeffrey L. Wrana,Karen Colwill,Frank Sicheri,Frank Sicheri,Samira Mubareka,Scott D. Gray-Owen,Steven J. Drews,Walter L. Siqueira,Miriam Barrios-Rodiles,Mario A. Ostrowski,Mario A. Ostrowski,James M. Rini,Yves Durocher,Allison McGeer,Allison McGeer,Allison McGeer,Jennifer L. Gommerman,Anne-Claude Gingras,Anne-Claude Gingras +43 more
TL;DR: It is confirmed that serum and saliva IgG antibodies to SARS-CoV-2 are maintained in the majority of COVID-19 patients for at least 3 months PSO, and IgG responses in saliva may serve as a surrogate measure of systemic immunity to Sars-Cov-2 based on their correlation with serum IgG responds.
Journal ArticleDOI
Naturally enhanced neutralizing breadth against SARS-CoV-2 one year after infection.
Zijun Wang,Frauke Muecksch,Dennis Schaefer-Babajew,Shlomo Finkin,Charlotte Viant,Christian Gaebler,Hans Heinrich Hoffmann,Christopher O. Barnes,Melissa Cipolla,Victor A. Ramos,Thiago Y. Oliveira,Alice Cho,Fabian Schmidt,Justin Da Silva,Eva Bednarski,Lauren C. Aguado,Jim Yee,Mridushi Daga,Martina Turroja,Katrina G. Millard,Mila Jankovic,Anna Gazumyan,Anna Gazumyan,Zhen Zhao,Charles M. Rice,Paul D. Bieniasz,Paul D. Bieniasz,Marina Caskey,Theodora Hatziioannou,Michel C. Nussenzweig,Michel C. Nussenzweig +30 more
TL;DR: In the absence of vaccination, antibody reactivity to the receptor binding domain (RBD) of SARS-CoV-2, neutralizing activity and the number of RBD-specific memory B cells remain relatively stable between 6 and 12 months after infection.
References
More filters
Journal ArticleDOI
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
TL;DR: The epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of patients with laboratory-confirmed 2019-nCoV infection in Wuhan, China, were reported.
Journal ArticleDOI
RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies.
TL;DR: This work presents some of the most notable new features and extensions of RAxML, such as a substantial extension of substitution models and supported data types, the introduction of SSE3, AVX and AVX2 vector intrinsics, techniques for reducing the memory requirements of the code and a plethora of operations for conducting post-analyses on sets of trees.
Journal ArticleDOI
A Novel Coronavirus from Patients with Pneumonia in China, 2019.
Na Zhu,Dingyu Zhang,Wenling Wang,Xingwang Li,Bo Yang,Jingdong Song,Xiang Zhao,Baoying Huang,Weifeng Shi,Roujian Lu,Peihua Niu,Faxian Zhan,Xuejun Ma,Dayan Wang,Wenbo Xu,Wenbo Xu,Guizhen Wu,George F. Gao,Wenjie Tan +18 more
TL;DR: Human airway epithelial cells were used to isolate a novel coronavirus, named 2019-nCoV, which formed a clade within the subgenus sarbecovirus, Orthocoronavirinae subfamily, which is the seventh member of the family of coronaviruses that infect humans.
Journal ArticleDOI
A pneumonia outbreak associated with a new coronavirus of probable bat origin
Peng Zhou,Xing-Lou Yang,Xian Guang Wang,Ben Hu,Lei Zhang,Wei Zhang,Hao Rui Si,Yan Zhu,Bei Li,Chao Lin Huang,Hui-Dong Chen,Jing Chen,Yun Luo,Hua Guo,Ren Di Jiang,Meiqin Liu,Ying Chen,Xu Rui Shen,Xi Wang,Xiao Shuang Zheng,Kai Zhao,Quanjiao Chen,Fei Deng,Lin Lin Liu,Bing Yan,Fa Xian Zhan,Yan-Yi Wang,Gengfu Xiao,Zhengli Shi +28 more
TL;DR: Identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China, and it is shown that this virus belongs to the species of SARSr-CoV, indicates that the virus is related to a bat coronav virus.
Journal ArticleDOI
SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TL;DR: It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.
Related Papers (5)
Convergent antibody responses to SARS-CoV-2 in convalescent individuals.
Davide F. Robbiani,Davide F. Robbiani,Christian Gaebler,Frauke Muecksch,Julio C. C. Lorenzi,Zijun Wang,Alice Cho,Marianna Agudelo,Christopher O. Barnes,Anna Gazumyan,Shlomo Finkin,Thomas Hagglof,Thiago Y. Oliveira,Charlotte Viant,Arlene Hurley,Hans Heinrich Hoffmann,Katrina G. Millard,Rhonda G. Kost,Melissa Cipolla,Kristie Gordon,Filippo Bianchini,Spencer T. Chen,Victor A. Ramos,Roshni Patel,Juan Dizon,Irina Shimeliovich,Pilar Mendoza,Harald Hartweger,Lilian Nogueira,Maggi Pack,Jill Horowitz,Fabian Schmidt,Yiska Weisblum,Eleftherios Michailidis,Alison W. Ashbrook,Eric Waltari,John E. Pak,Kathryn E. Huey-Tubman,Nicholas Koranda,Pauline R. Hoffman,Anthony P. West,Charles M. Rice,Theodora Hatziioannou,Pamela J. Bjorkman,Paul D. Bieniasz,Paul D. Bieniasz,Marina Caskey,Michel C. Nussenzweig,Michel C. Nussenzweig +48 more
Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model.
Thomas F. Rogers,Thomas F. Rogers,Fangzhu Zhao,Deli Huang,Nathan Beutler,Alison Burns,Wan-ting He,Oliver Limbo,Oliver Limbo,Chloe Smith,Ge Song,Jordan L. Woehl,Jordan L. Woehl,Linlin Yang,Robert K. Abbott,Robert K. Abbott,Sean Callaghan,Elijah Garcia,Jonathan Hurtado,Mara Parren,Linghang Peng,Sydney I. Ramirez,James Ricketts,Michael J. Ricciardi,Stephen A. Rawlings,Nicholas C. Wu,Meng Yuan,Davey M. Smith,David Nemazee,John R. Teijaro,James E. Voss,Ian A. Wilson,Raiees Andrabi,Bryan Briney,Elise Landais,Devin Sok,Joseph G. Jardine,Joseph G. Jardine,Dennis R. Burton +38 more
Potent neutralizing antibodies against multiple epitopes on SARS-CoV-2 spike.
Lihong Liu,Pengfei Wang,Manoj S. Nair,Jian Yu,Micah Rapp,Qian Wang,Yang Luo,Jasper Fuk-Woo Chan,Jasper Fuk-Woo Chan,Vincent Sahi,Amir Figueroa,Xinzheng V. Guo,Gabriele Cerutti,Jude Bimela,Jason Gorman,Tongqing Zhou,Zhiwei Chen,Zhiwei Chen,Kwok-Yung Yuen,Kwok-Yung Yuen,Peter D. Kwong,Peter D. Kwong,Joseph Sodroski,Michael T. Yin,Zizhang Sheng,Zizhang Sheng,Yaoxing Huang,Lawrence Shapiro,Lawrence Shapiro,David D. Ho +29 more