Journal ArticleDOI
LTP promotes formation of multiple spine synapses between a single axon terminal and a dendrite
TLDR
In this paper, the morphology of synapses activated by high-frequency stimulation and identified by accumulated calcium in dendritic spines was analyzed using electron microscopy to identify the formation of new synapses contacting the same presynaptic terminal.Abstract:
Structural remodelling of synapses and formation of new synaptic contacts has been postulated as a possible mechanism underlying the late phase of long-term potentiation (LTP), a form of plasticity which is involved in learning and memory Here we use electron microscopy to analyse the morphology of synapses activated by high-frequency stimulation and identified by accumulated calcium in dendritic spines LTP induction resulted in a sequence of morphological changes consisting of a transient remodelling of the postsynaptic membrane followed by a marked increase in the proportion of axon terminals contacting two or more dendritic spines Three-dimensional reconstruction revealed that these spines arose from the same dendrite As pharmacological blockade of LTP prevented these morphological changes, we conclude that LTP is associated with the formation of new, mature and probably functional synapses contacting the same presynaptic terminal and thereby duplicating activated synapsesread more
Citations
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Posted ContentDOI
Multi-synaptic boutons are a feature of CA1 hippocampal connections that may underlie network synchrony
Mark Rigby,Federico W. Grillo,Benjamin Compans,Guilherme Neves,Júlia V. Gallinaro,Sophie Nashashibi,Gema Vizcay-Barrena,Florian Levet,Jean-Baptiste Sibarita,Angus Kirkland,Roland A. Fleck,Claudia Clopath,Juan Burrone +12 more
TL;DR: Curiously, synaptic properties such as active zone (AZ) or postsynaptic density (PSD) size exhibited less within-MSB variation when compared to neighbouring SSBs, features that were confirmed by super-resolution light microscopy and computer simulations suggest that these properties favour synchronous activity in CA1 networks.
Book ChapterDOI
Activity-Mediated Structural Plasticity of Dendritic Spines
TL;DR: Brain activity associated with behavioral learning paradigms results in two main structural reorganizations of synapses that result in long-lasting circuit rearrangements that underlie learning and memory processes and could represent important pathophysiological targets of mechanisms implicated in brain diseases.
Journal ArticleDOI
Induction of synapse formation by de novo neurotransmitter synthesis
Scott R. Burlingham,Nicole F. Wong,Lindsay Peterkin,Lily Lubow,Carolina dos Santos Passos,Orion Benner,Michael Ghebrial,Thomas Cast,Matthew A. Xu-Friedman,Thomas C. Südhof,Soham Chanda +10 more
TL;DR: In this article , the role of neurotransmitters in the assembly of postsynapses is investigated, and it is shown that ectopic expression of GABA-synthesis enzymes and vesicular transporters is sufficient to both produce GABA from ambient glutamate and transmit it from presynaptic terminals.
Alcohol alters the expression of Soluble N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptors (SNAREs) and spontaneous γ-Aminobutyric Acid (GABA) release...
TL;DR: Investigation of the effects of acute alcohol on the expression of genes encoding for synaptic vesicle fusion machinery proteins that form the soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) complex found that ethanol treatment of mouse cortical neurons increased the mRNA and protein expression levels of a subset of SNARE complex proteins.
References
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Journal ArticleDOI
A synaptic model of memory: long-term potentiation in the hippocampus
TL;DR: The best understood form of long-term potentiation is induced by the activation of the N-methyl-d-aspartate receptor complex, which allows electrical events at the postsynaptic membrane to be transduced into chemical signals which, in turn, are thought to activate both pre- and post Synaptic mechanisms to generate a persistent increase in synaptic strength.
Journal ArticleDOI
Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale
TL;DR: DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions.
Journal ArticleDOI
A simple method for organotypic cultures of nervous tissue
TL;DR: Hippocampal slices prepared from 2-23-day-old neonates were maintained in culture at the interface between air and a culture medium and yielded thin slices which remain 1-4 cell layers thick and are characterized by a well preserved organotypic organization.
Journal ArticleDOI
The unbiased estimation of number and sizes of arbitrary particles using the disector
TL;DR: A three‐dimensional counting rule and its integral test system, the disector, for obtaining unbiased estimates of the number of arbitrary particles in a specimen is presented.
PatentDOI
Dissecting the regulatory circuitry of a eukaryotic genome
TL;DR: The results reveal an unanticipated level of regulation which is superimposed on that due to gene-specific transcription factors, a novel mechanism for coordinate regulation of specific sets of genes when cells encounter limiting nutrients, and evidence that the ultimate targets of signal transduction pathways can be identified within the initiation apparatus.
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Dendritic spine changes associated with hippocampal long-term synaptic plasticity
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