Journal ArticleDOI
LTP promotes formation of multiple spine synapses between a single axon terminal and a dendrite
TLDR
In this paper, the morphology of synapses activated by high-frequency stimulation and identified by accumulated calcium in dendritic spines was analyzed using electron microscopy to identify the formation of new synapses contacting the same presynaptic terminal.Abstract:
Structural remodelling of synapses and formation of new synaptic contacts has been postulated as a possible mechanism underlying the late phase of long-term potentiation (LTP), a form of plasticity which is involved in learning and memory Here we use electron microscopy to analyse the morphology of synapses activated by high-frequency stimulation and identified by accumulated calcium in dendritic spines LTP induction resulted in a sequence of morphological changes consisting of a transient remodelling of the postsynaptic membrane followed by a marked increase in the proportion of axon terminals contacting two or more dendritic spines Three-dimensional reconstruction revealed that these spines arose from the same dendrite As pharmacological blockade of LTP prevented these morphological changes, we conclude that LTP is associated with the formation of new, mature and probably functional synapses contacting the same presynaptic terminal and thereby duplicating activated synapsesread more
Citations
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Studying the protein organization of the postsynaptic density by a novel solid phase- and chemical cross-linking-based technology.
TL;DR: A model for the molecular organization of the postsynaptic density (PSD) is proposed and it is suggested that the N-methyl-d-aspartate receptors and the α-subunits of calcium/calmodulin-dependent protein kinase II are likely to reside on the surface of the PSD although with unique local protein organizations.
Journal ArticleDOI
Molecules involved in the formation of synaptic connections in muscle and brain.
TL;DR: The study of how synapses are formed during development is of relevance for the understanding of the cellular and molecular processes involved in learning and memory.
Journal ArticleDOI
Fine structure of synapses on dendritic spines
TL;DR: Recent studies on the fine structure of spines and synapses using high-pressure freezing (HPF), which avoids protein denaturation by aldehydes and results in an excellent preservation of ultrastructural detail, establish HPF as an appropriate method for studying thefine structure and molecular composition of synapses on dendritic spines.
Research report Ischemia-induced increase in long-term potentiation is warded off by specific calpain inhibitor PD150606
TL;DR: In this article, the effect of specific, membrane-permeable calpain inhibitor, PD150606, was analysed on synaptic efficacy in in vitro brain slices experiments after ischemic insult of rats in vivo, and on cell viability in a glutamate excitotoxicity test in mouse cell culture.
Journal ArticleDOI
Long-term synaptic morphometry changes after induction of long-term potentiation and long-term depression in the dentate gyrus of awake rats are not simply mirror phenomena.
Szilvia Mezey,Valérie Doyère,Ian E. J. De Souza,E. Harrison,Karine Cambon,Claire E Kendal,Heather A. Davies,Serge Laroche,Michael G. Stewart +8 more
TL;DR: It is found that LTP of the medial, but not lateral, perforant path is associated with a specific increase in perforated axospinous synapses in the potentiated area of awake rats, and heterosynaptic LTD isassociated with an input‐specific increase in axodendritic synapse density.
References
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The unbiased estimation of number and sizes of arbitrary particles using the disector
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Dissecting the regulatory circuitry of a eukaryotic genome
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