Journal ArticleDOI
LTP promotes formation of multiple spine synapses between a single axon terminal and a dendrite
TLDR
In this paper, the morphology of synapses activated by high-frequency stimulation and identified by accumulated calcium in dendritic spines was analyzed using electron microscopy to identify the formation of new synapses contacting the same presynaptic terminal.Abstract:
Structural remodelling of synapses and formation of new synaptic contacts has been postulated as a possible mechanism underlying the late phase of long-term potentiation (LTP), a form of plasticity which is involved in learning and memory Here we use electron microscopy to analyse the morphology of synapses activated by high-frequency stimulation and identified by accumulated calcium in dendritic spines LTP induction resulted in a sequence of morphological changes consisting of a transient remodelling of the postsynaptic membrane followed by a marked increase in the proportion of axon terminals contacting two or more dendritic spines Three-dimensional reconstruction revealed that these spines arose from the same dendrite As pharmacological blockade of LTP prevented these morphological changes, we conclude that LTP is associated with the formation of new, mature and probably functional synapses contacting the same presynaptic terminal and thereby duplicating activated synapsesread more
Citations
More filters
Journal ArticleDOI
The neurofilament infrastructure of a developing presynaptic calyx.
TL;DR: It is found that labeling the calyx membranes with a lipophilic dye delivered by diffusion down the preganglionic nerve reveals a large membrane structure engulfing the postsynaptic cell by the end of embryogenesis, similar to that seen when presynaptic neurofilament bundles are labeled with antibodies selective for highly phosphorylated neurofilaments.
Journal ArticleDOI
Role of Ca2+/calmodulin-dependent protein kinase II in dendritic spine remodeling during epileptiform activity in vitro.
TL;DR: Data show at least two roles for CaMKII activity in maintenance and remodeling of dendritic spines under basal or EA conditions, apparently by promoting translocation of PSDs from dendrite shafts to spines and/or selectively stabilizing spine rather than shaft PSDs.
Journal ArticleDOI
Rapid and long-lasting increase in sites for synapse assembly during late-phase potentiation in rat hippocampal neurons
TL;DR: Examination of changes in pre- and postsynaptic puncta and structures during glutamate-induced late-phase potentiation in cultured hippocampal neurons finds new sites could contribute to the transition between the early and late phase mechanisms of plasticity by serving as seeds for the formation and maintenance of new synapses, thus acting as local “tags” for protein synthesis-dependent synaptic growth during late- phase plasticity.
Journal ArticleDOI
Synaptic loss and retention of different classic cadherins with LTP-associated synaptic structural remodeling in vivo.
George W. Huntley,Alice Elste,Shekhar B. Patil,Ozlem Bozdagi,Deanna L. Benson,Oswald Steward +5 more
TL;DR: It is found that the density and size of cadherin‐8 puncta are significantly diminished in the potentiated middle molecular layer (MML) while concurrently, N‐cadherin remains tightly clustered at remodeled synapses, suggesting that activity‐regulated loss and retention of different synaptic cadherins could contribute to dual demands of both flexibility and stability in synapse structure.
Journal ArticleDOI
Dynamic aspects of cadherin-mediated adhesion in synapse development and plasticity.
TL;DR: Cadherins are calcium‐dependent cell adhesion molecules that are enriched at synapses that indicate important functional roles in synaptic targeting during brain development, and in regulating functional and structural aspects of synaptic plasticity and neural repair throughout life.
References
More filters
Journal ArticleDOI
A synaptic model of memory: long-term potentiation in the hippocampus
TL;DR: The best understood form of long-term potentiation is induced by the activation of the N-methyl-d-aspartate receptor complex, which allows electrical events at the postsynaptic membrane to be transduced into chemical signals which, in turn, are thought to activate both pre- and post Synaptic mechanisms to generate a persistent increase in synaptic strength.
Journal ArticleDOI
Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale
TL;DR: DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions.
Journal ArticleDOI
A simple method for organotypic cultures of nervous tissue
TL;DR: Hippocampal slices prepared from 2-23-day-old neonates were maintained in culture at the interface between air and a culture medium and yielded thin slices which remain 1-4 cell layers thick and are characterized by a well preserved organotypic organization.
Journal ArticleDOI
The unbiased estimation of number and sizes of arbitrary particles using the disector
TL;DR: A three‐dimensional counting rule and its integral test system, the disector, for obtaining unbiased estimates of the number of arbitrary particles in a specimen is presented.
PatentDOI
Dissecting the regulatory circuitry of a eukaryotic genome
TL;DR: The results reveal an unanticipated level of regulation which is superimposed on that due to gene-specific transcription factors, a novel mechanism for coordinate regulation of specific sets of genes when cells encounter limiting nutrients, and evidence that the ultimate targets of signal transduction pathways can be identified within the initiation apparatus.
Related Papers (5)
Dendritic spine changes associated with hippocampal long-term synaptic plasticity
Florian Engert,Tobias Bonhoeffer +1 more