Journal ArticleDOI
LTP promotes formation of multiple spine synapses between a single axon terminal and a dendrite
TLDR
In this paper, the morphology of synapses activated by high-frequency stimulation and identified by accumulated calcium in dendritic spines was analyzed using electron microscopy to identify the formation of new synapses contacting the same presynaptic terminal.Abstract:
Structural remodelling of synapses and formation of new synaptic contacts has been postulated as a possible mechanism underlying the late phase of long-term potentiation (LTP), a form of plasticity which is involved in learning and memory Here we use electron microscopy to analyse the morphology of synapses activated by high-frequency stimulation and identified by accumulated calcium in dendritic spines LTP induction resulted in a sequence of morphological changes consisting of a transient remodelling of the postsynaptic membrane followed by a marked increase in the proportion of axon terminals contacting two or more dendritic spines Three-dimensional reconstruction revealed that these spines arose from the same dendrite As pharmacological blockade of LTP prevented these morphological changes, we conclude that LTP is associated with the formation of new, mature and probably functional synapses contacting the same presynaptic terminal and thereby duplicating activated synapsesread more
Citations
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Dendritic spines: structure, dynamics and regulation.
Heike Hering,Morgan Sheng +1 more
TL;DR: New insights into the molecular mechanisms that regulate spine morphogenesis offer potential ways to manipulate dendritic spines in vivo and to explore their physiological roles in the brain.
Journal ArticleDOI
Structural plasticity and memory.
TL;DR: Rapid changes in cytoskeletal and adhesion molecules after learning contribute to short-term plasticity and memory, whereas later changes, which depend on de novo protein synthesis as well as the early modifications, seem to be required for the persistence of long-term memory.
Journal ArticleDOI
Reconstruct: a free editor for serial section microscopy
TL;DR: Reconstruct is a free editor designed to facilitate montaging, alignment, analysis and visualization of serial sections, which can reduce the time and resources expended for serial section studies and allows a larger tissue volume to be analysed more quickly.
Journal ArticleDOI
Balancing Structure and Function at Hippocampal Dendritic Spines
TL;DR: Advances in imaging and computing technologies may provide the resources needed to reconstruct entire neural circuits as well as having sufficient resolution to determine the extrinsic factors and the intrinsic factors that are required to build and maintain synapses.
Journal ArticleDOI
Actin-based plasticity in dendritic spines.
TL;DR: New developments in light microscopy allow changes in spine morphology to be directly visualized in living neurons and suggest that a common mechanism, based on dynamic actin filaments, is involved in both the formation of dendritic spines during development and their structural plasticity at mature synapses.
References
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