Journal ArticleDOI
LTP promotes formation of multiple spine synapses between a single axon terminal and a dendrite
TLDR
In this paper, the morphology of synapses activated by high-frequency stimulation and identified by accumulated calcium in dendritic spines was analyzed using electron microscopy to identify the formation of new synapses contacting the same presynaptic terminal.Abstract:
Structural remodelling of synapses and formation of new synaptic contacts has been postulated as a possible mechanism underlying the late phase of long-term potentiation (LTP), a form of plasticity which is involved in learning and memory Here we use electron microscopy to analyse the morphology of synapses activated by high-frequency stimulation and identified by accumulated calcium in dendritic spines LTP induction resulted in a sequence of morphological changes consisting of a transient remodelling of the postsynaptic membrane followed by a marked increase in the proportion of axon terminals contacting two or more dendritic spines Three-dimensional reconstruction revealed that these spines arose from the same dendrite As pharmacological blockade of LTP prevented these morphological changes, we conclude that LTP is associated with the formation of new, mature and probably functional synapses contacting the same presynaptic terminal and thereby duplicating activated synapsesread more
Citations
More filters
Journal ArticleDOI
The postsynaptic density.
TL;DR: The characterization of synapse/PSD proteins is especially important in the light of recent data suggesting that several mental disorders have their molecular defect at the synapse /PSD level.
Journal ArticleDOI
Stages of synapse development defined by dependence on F-actin.
Wandong Zhang,Deanna L. Benson +1 more
TL;DR: It is demonstrated that F-actin is essential for the development and maintenance of young synapses and proposed that it may be a principal target for stabilizing or destabilizing signals that ultimately result in synapse maintenance or elimination.
Journal ArticleDOI
Endosomal compartments serve multiple hippocampal dendritic spines from a widespread rather than a local store of recycling membrane.
TL;DR: Serial electron microscopy was used to ascertain the morphology and distribution of all membranous intracellular compartments in distal dendrites of hippocampal CA1 pyramidal neurons in juvenile and adult rats and revealed that up to 20 spines shared a recycling pool of plasmalemmal proteins rather than maintaining independent stores at each spine.
Journal ArticleDOI
Structural Synaptic Modifications Associated with Hippocampal LTP and Behavioral Learning
TL;DR: It appears, therefore, that cellular mechanisms of learning and memory are likely to include both synaptogenesis and synapse remodeling, including the conversion of 'silent' synapses into functional synapses.
Journal ArticleDOI
Rapid Dendritic Remodeling in the Developing Retina: Dependence on Neurotransmission and Reciprocal Regulation by Rac and Rho
TL;DR: It is suggested that the Rho family of small GTPases could integrate activity-dependent and -independent signals from afferents, thereby adjusting target motility and maximizing the chance for initial contact and subsequent synaptogenesis.
References
More filters
Journal ArticleDOI
A synaptic model of memory: long-term potentiation in the hippocampus
TL;DR: The best understood form of long-term potentiation is induced by the activation of the N-methyl-d-aspartate receptor complex, which allows electrical events at the postsynaptic membrane to be transduced into chemical signals which, in turn, are thought to activate both pre- and post Synaptic mechanisms to generate a persistent increase in synaptic strength.
Journal ArticleDOI
Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale
TL;DR: DNA microarrays containing virtually every gene of Saccharomyces cerevisiae were used to carry out a comprehensive investigation of the temporal program of gene expression accompanying the metabolic shift from fermentation to respiration, and the expression patterns of many previously uncharacterized genes provided clues to their possible functions.
Journal ArticleDOI
A simple method for organotypic cultures of nervous tissue
TL;DR: Hippocampal slices prepared from 2-23-day-old neonates were maintained in culture at the interface between air and a culture medium and yielded thin slices which remain 1-4 cell layers thick and are characterized by a well preserved organotypic organization.
Journal ArticleDOI
The unbiased estimation of number and sizes of arbitrary particles using the disector
TL;DR: A three‐dimensional counting rule and its integral test system, the disector, for obtaining unbiased estimates of the number of arbitrary particles in a specimen is presented.
PatentDOI
Dissecting the regulatory circuitry of a eukaryotic genome
TL;DR: The results reveal an unanticipated level of regulation which is superimposed on that due to gene-specific transcription factors, a novel mechanism for coordinate regulation of specific sets of genes when cells encounter limiting nutrients, and evidence that the ultimate targets of signal transduction pathways can be identified within the initiation apparatus.
Related Papers (5)
Dendritic spine changes associated with hippocampal long-term synaptic plasticity
Florian Engert,Tobias Bonhoeffer +1 more