Native phasing of x-ray free-electron laser data for a G protein-coupled receptor.
Alexander Batyuk,Lorenzo Galli,Andrii Ishchenko,Gye Won Han,Cornelius Gati,Petr Popov,Petr Popov,Ming-Yue Lee,Benjamin Stauch,Thomas A. White,Anton Barty,Andrew Aquila,Mark S. Hunter,Mengning Liang,Sébastien Boutet,Mengchen Pu,Zhi-Jie Liu,Zhi-Jie Liu,Garrett Nelson,Daniel James,Chufeng Li,Yun Zhao,John C. H. Spence,Wei Liu,Petra Fromme,Vsevolod Katritch,Uwe Weierstall,Raymond C. Stevens,Vadim Cherezov +28 more
TLDR
The ability to solve the crystallographic phase problem for SFX data collected with an XFEL using the anomalous signal from native sulfur atoms is demonstrated, leading to a bias-free room temperature structure of the human A2A adenosine receptor at 1.9 Å resolution.Abstract:
Serial femtosecond crystallography (SFX) takes advantage of extremely bright and ultrashort pulses produced by x-ray free-electron lasers (XFELs), allowing for the collection of high-resolution diffraction intensities from micrometer-sized crystals at room temperature with minimal radiation damage, using the principle of “diffraction-before-destruction.” However, de novo structure factor phase determination using XFELs has been difficult so far. We demonstrate the ability to solve the crystallographic phase problem for SFX data collected with an XFEL using the anomalous signal from native sulfur atoms, leading to a bias-free room temperature structure of the human A2A adenosine receptor at 1.9 A resolution. The advancement was made possible by recent improvements in SFX data analysis and the design of injectors and delivery media for streaming hydrated microcrystals. This general method should accelerate structural studies of novel difficult-to-crystallize macromolecules and their complexes.read more
Citations
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Journal ArticleDOI
Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons.
Tobias Weinert,Natacha Olieric,Robert K. Y. Cheng,Steffen Brünle,Daniel James,Dmitry Ozerov,Dardan Gashi,Laura Vera,May Marsh,Kathrin Jaeger,Florian S. N. Dworkowski,Ezequiel Panepucci,Shibom Basu,Petr Skopintsev,Andrew S. Doré,T. Geng,Robert M. Cooke,Mengning Liang,Andrea E. Prota,Valerie Panneels,Przemyslaw Nogly,Ulrich Ermler,Gebhard F. X. Schertler,Gebhard F. X. Schertler,Michael Hennig,Michel O. Steinmetz,Michel O. Steinmetz,Meitian Wang,Jörg Standfuss +28 more
TL;DR: It is demonstrated that serial millisecond crystallography at a synchrotron beamline equipped with high-viscosity injector and high frame-rate detector allows typical crystallographic experiments to be performed at room-temperature.
Journal ArticleDOI
XFELs for structure and dynamics in biology
TL;DR: The use of X-ray lasers to obtain structures and molecular movies in biology is reviewed and methods include single-particle imaging, serial crystallography and fast solution scattering at room temperature.
Journal ArticleDOI
Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation
Jose M. Martin-Garcia,Chelsie E. Conrad,Garrett Nelson,Natasha Stander,Nadia A. Zatsepin,James Zook,Lan Zhu,James Geiger,Eugene Chun,David J. Kissick,Mark Hilgart,Craig M. Ogata,Andrii Ishchenko,Nirupa Nagaratnam,Shatabdi Roy-Chowdhury,Jesse Coe,Ganesh Subramanian,Alexander Schaffer,Daniel James,Gihan Ketwala,Nagarajan Venugopalan,S. Xu,Stephen Corcoran,Dale Ferguson,Uwe Weierstall,John C. H. Spence,Vadim Cherezov,Petra Fromme,Robert F. Fischetti,Wei Liu +29 more
TL;DR: The first high-viscosity injector-based SMX experiments carried out at a US synchrotron source, the Advanced Photon Source (APS), are reported.
Journal ArticleDOI
A Bright Future for Serial Femtosecond Crystallography with XFELs
TL;DR: The latest developments in instrumentation, sample delivery, data analysis, crystallization methods, and applications of SFX to important biological questions are reviewed, and brief insights into the bright future of structural biology using XFELs are provided.
References
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Phenix - a comprehensive python-based system for macromolecular structure solution
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