Aligarh Muslim University

About: Aligarh Muslim University is a education organization based out in Aligarh, Uttar Pradesh, India. It is known for research contribution in the topics: Population & Adsorption. The organization has 8218 authors who have published 16416 publications receiving 289068 citations. The organization is also known as: AMU.

Strand scission in DNA induced by quercetin and Cu(II): role of Cu(I) and oxygen free radicals.

Abstract: The naturally occurring flavonoid, quercetin, in the presence of Cu(II) and molecular oxygen caused breakage of calf thymus DNA, supercoiled pBR322 plasmid DNA and single-stranded M13 phage DNA. In the case of the plasmid, the product(s) were relaxed circles or a mixture of these and linear molecules depending upon the conditions. For the breakage reaction, Cu(II) could be replaced by Fe(III) but not by other ions tested [Fe(II), Co(II), Ni(II) and Ca(II)]. Structurally related flavonoids, rutin, galangin, apigenin and fisetin, were ineffective or less effective than quercetin in causing DNA breakage. In the case of the quercetin--Cu(II) reaction, Cu(I) was shown to be an essential intermediate by using the Cu(I)-sequestering reagents, neocuproine and bathocuproine. By using Job plots we established that, in the absence of DNA, five Cu(II) ions can be reduced by one quercetin molecule; in contrast, two ions were reduced per quercetin molecule in the DNA breakage reaction. Equally neocuproine inhibited the DNA breakage reaction. The involvement of active oxygen in the reaction was established by the inhibition of DNA breakage by superoxide dismutase, iodide, mannitol, formate and catalase (the inhibition was complete in the last case). From these data we propose a mechanism for the DNA strand scission reaction of quercetin and related flavonoids.

Azimuthal anisotropy of D-meson production in Pb-Pb collisions at √sNN=2.76 TeV

Abstract: The production of the prompt charmed mesons D0, D+, and D*+ relative to the reaction plane was measured in Pb-Pb collisions at a center-of-mass energy per nucleon-nucleon collision of √sNN=2.76TeV with the ALICE detector at the CERN Large Hadron Collider. D mesons were reconstructed via their hadronic decays at central rapidity in the transverse-momentum (p_T) interval 2–16 GeV/c. The azimuthal anisotropy is quantified in terms of the second coefficient v2 in a Fourier expansion of the D-meson azimuthal distribution and in terms of the nuclear modification factor R_AA, measured in the direction of the reaction plane and orthogonal to it. The v2 coefficient was measured with three different methods and in three centrality classes in the interval 0%–50%. A positive v2 is observed in midcentral collisions (30%–50% centrality class), with a mean value of 0.204+0.099−0.036 (tot. unc.) in the interval 2

Mycofabricated biosilver nanoparticles interrupt Pseudomonas aeruginosa quorum sensing systems.

Abstract: Quorum sensing (QS) is a chemical communication process that Pseudomonas aeruginosa uses to regulate virulence and biofilm formation. Disabling of QS is an emerging approach for combating its pathogenicity. Silver nanoparticles (AgNPs) have been widely applied as antimicrobial agents against human pathogenic bacteria and fungi, but not for the attenuation of bacterial QS. Here we mycofabricated AgNPs (mfAgNPs) using metabolites of soil fungus Rhizopus arrhizus BRS-07 and tested their effect on QS-regulated virulence and biofilm formation of P. aeruginosa. Transcriptional studies demonstrated that mfAgNPs reduced the levels of LasIR-RhlIR. Treatment of mfAgNPs inhibited biofilm formation, production of several virulence factors (e.g. LasA protease, LasB elastrase, pyocyanin, pyoverdin, pyochelin, rhamnolipid, and alginate) and reduced AHLs production. Further genes quantification analyses revealed that mfAgNPs significantly down-regulated QS-regulated genes, specifically those encoded to the secretion of virulence factors. The results clearly indicated the anti-virulence property of mfAgNPs by inhibiting P. aeruginosa QS signaling.

Phosphate-solubilizing bacteria-assisted phytoremediation of metalliferous soils: a review

Abstract: Heavy metal pollution of soils is of great concern. The presence of the toxic metal species above critical concentration not only harmfully affects human health but also the environment. Among existing strategies to remediate metal contaminates in soils, phytoremediation approach using metal accumulating plants is much convincing in terms of metal removal efficiency, but it has many limitations because of slow plant growth and decreased biomass owing to metal-induced stress. In addition, constrain of metal bioavailability in soils is the prime factor to restrict its applicability. Phytoremediation of metals in association with phosphate-solubilizing bacteria (PSB) considerably overcomes the practical drawbacks imposed by metal stress on plants. This review is an effort to describe mechanism of PSB in supporting and intensifying phytoremediation of heavy metals in soils and to address the developmental status of the current trend in application of PSB in this context.

Broad-spectrum antibacterial and antifungal properties of certain traditionally used Indian medicinal plants

Abstract: Ethanolic extracts of 22 traditionally used Indian medicinal plants were studied for their antimicrobial activity against seven bacteria (Staphylococcus aureus, Salmonella typhimurium, S. paratyphi, S. typhi, E. coli, Shigella dysenteriae and Pseudomonas aeruginosa) and five filamentous fungi (Aspergillus niger, Alternaria alternata, Fusarium chlamydosporum, Rhizoctonia bataticola and Trichoderma viride) and a yeast Candida albicans of clinical origin. Of these, 16 plant extracts showed varied level of antibacterial activity against one or more test bacteria. Similarly antifungal and anticandidal activity was detected among 17 and 9 plant extracts respectively. Broad-spectrum antimicrobial activity (both antibacterial and antifungal) was detected among crude extracts of Bryophyllum pinnatum (leaves), Caesalpinia bonducella (seeds), Delonix regia (flower), Hedychium spicatum (fruits), Mangifera indica (leaves), Murraya coenigii (leaves) and Syzgium cumini (seeds). Similarly extracts of Cichorium intybus (roots), Ficus religiosa (leaves) and Trigonella foenum-graecum (leaves) demonstrated more antibacterial activity with less antifungal activity. On the other hand Pistacia integerrima (stems) and Rheum emodi (roots) demonstrated more antifungal activity with less antibacterial activity.